乐尔脉胶囊对大鼠缺血再灌注后期大脑皮层细胞凋亡的干预作用

目的:研究乐尔脉胶囊(LEM)对大鼠局灶性脑缺血2h再灌注30d后所致大脑皮层神经细胞凋亡的干预作用。方法:以线栓阻断(MCAO)法制备大鼠右侧大脑中动脉缺血再灌注模型,分为假手术、模型、盐酸氟桂利嗪及LEM高、低剂量组;应用免疫组化、凋亡细胞原位末端标记法(TUNEL)与逆转录聚合酶链反应(RT-PCR)技术检测大鼠大脑细胞凋亡和细胞凋亡相关基因产物(Fas)、凋亡促进基因(Bax)mRNA的表达,并进行图像分析。结果:模型组凋亡细胞主要位于缺血侧大脑皮层缺血边缘区(半暗带区);缺血侧大脑皮层Fas、Bax mRNA的表达在缺血再灌注30d后仍有升高;LEM组Fas、Bax mRNA的表达显著低于模型组(P<0.01),凋亡细胞数也显著低于模型组(P<0.01)。LEM组可明显降低损伤侧脑组织Fas、Bax mRNA的表达,抑制细胞凋亡,减轻缺血再灌注对大鼠大脑皮层神经细胞的损伤。结论:LEM对大鼠脑缺血再灌注30d后的细胞凋亡有一定的干预作用。

Effect of Leermai Capsules on Pallium Neural Apoptosis Following Late Stage Cerebral Ischemia Reper fusion Injury in Rats

OBJECTIVE:To observe the effect of Leermai capsules(LEM) on pallium neural apoptosis in rats after focal cerebral ischemia for 2 h and reperfusion for 30 days.METHODS:The models of focal cerebral ischemia reperfusion were established rats by a method of middle cerebral artery occlusion(MCAO).The adult rats were randomly divided into sham-oper-ated group,model group,Flunarizine hydrochloride-treated group,the LEM-treated group(high and low doses respectively).The expression of Fas,Bax mRNA and rats' neural apoptosis were assessed by immunohistochemistry staining,TUNEL and RT-PCR,and the image analysis was conducted as well.RESULTS:The apoptotic cells of the model group were mainly located in the ischemic margin(penumbra) of the ischemic pallium;and the expression of Fas,Bax mRNA remained increased after cerebral ischemia reperfusion for 30 days.Compared with the model group,the expression of Fas,Bax mRNA proteins and the number of the apoptosis cells in LEM-treated group were significantly lower(P<0.01).LEM obviously reduced the expression of Fas and Bax mRNA in the injured side of brain,inhibited apoptosis and lessened damage of neural cells in pallium of rats after cerebral ischemia reperfusion.CONCLUSION:LEM has certain interventional effect on neural apoptosis in rats after focal cerebral ischemia reperfusion for 30 days.