结核杆菌复苏促进因子在分枝杆菌培养中的作用观察

目的 探讨结核杆菌复苏因子(TB-RPF)在结核患者标本的分枝杆菌培养中的作用.方法 收集疑似结核患者的痰液42例,支冲液8例,胸腔积液25例,经4%NaOH液化处理后用PBS洗涤并悬浮菌体,每份标本分设对照组和RPF组,按照两组各0.5 ml菌液量接种于MGIT培养管.对照组培养基采用含Middlebrook7H9的...

The role of TB-resuscitation promoting factor in culture of mycobacterium tuberculosis

Objective To explore the role of TB-resuscitation promoting factor proteins ( TB-RPFs )in culture of mycobacterium tuberculosis. Methods 42 sputum samples, 8 samples of bronchoalveolar lavage fluid ( BALF ) and 25 samples of pleural fluid for tuberculosis patients were collected .All samples were treated with 4%NaOH, washed with PBS and then suspended. Each sample was divided into control group and RPF group. Control group was cultured in MGIT, RPF group was cultured with five types of TB-RPFs in MGIT. All samples were cultured in the automated mycobacteria testing M960 system.The cultivation time for positive cultures was recorded and the positive cultures were used to identify mycobacterium strains. Results The positive rate of sputum culture was 42.86% in the RPF group and 45.24% in the control group; that of BALF was 37.5% and 62.5%, respectively; and the positive rate of pleural fluid was 20% in both groups. There were no significant differences between the two groups. Of all the positive samples, 9 had the same days for positive results.6 samples ( 3 pleural fluid, 2 sputum, and 1 BALF ) were reported positive earlier in the RPF group than the control group, with an average length of 4.33 days but,9 were reported positive later in the RPF group group with an average length of 1.11 days. 3 samples were cultured positive in the control group but negative in the RPF group.Conclusions There is no enough evidence to prove that TB-RPFs can improve the positive rate for mycobacterium culture in sputum, BALF and pleural fluid samples. But TB-RPFs significantly shorten the positive culture time in pleural fluid. The role of TB-RPFs for culturing mycobacterium in different samples needs to be further studied.