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| 鼻咽癌细胞肝转移亚株的建立 | |
| 引用本文： | 韩春,王爽,刘莉,姚开泰. 鼻咽癌细胞肝转移亚株的建立[J]. 南方医科大学学报, 2006, 26(5): 654-657 |
| 作者姓名： | 韩春 王爽 刘莉 姚开泰 |
| 作者单位： | 南方医科大学病理教研室,广东,广州,510515;南方医科大学病理教研室,广东,广州,510515;南方医科大学病理教研室,广东,广州,510515;南方医科大学病理教研室,广东,广州,510515 |
| 摘 要： | 目的 建立鼻咽癌细胞肝转移亚株，研究鼻咽癌肝转移机制。方法 利用裸鼠体内传代的方法获得肝转移细胞亚株，并用体内和体外实验来观察其侵袭转移能力。结果 经体内反复筛选得到肝转移能力高的亚株5-8F-H3B-EGFP．其侵袭性、运动性以及形成肝转移灶的能力均大于母系5-8F-EGFP，并具有较高的肝脏亲和性。结论 该亚株的建立为今后研究鼻咽癌肝转移的分子机制奠定了基础。 |
| 关 键 词： | 鼻咽癌 肝转移 肿瘤侵袭 细胞亚株 |
| 文章编号： | 1673-4254（2006）05-0654-04 |
| 收稿时间： | 2005-11-16 |
| 修稿时间： | 2005-11-16 |
Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line |
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| HAN Chun,WANG Shuang,LIU Li,YAO Kai-tai. Establishment of hepatic metastatic subline of nasopharyngeal carcinoma cell line[J]. Journal of Southern Medical University, 2006, 26(5): 654-657 | |
| Authors: | HAN Chun WANG Shuang LIU Li YAO Kai-tai |
| Affiliation: | Department of Pathology, Southern Medical University, Guangzhou 510515, China. xiaochun@fimmu.com |
| Abstract: | Objective To establish a hepatic metastatic subline of nasopharyngeal carcinoma(NPC)cell line.Methods NPC cells metastatic to the liver were isolated from nude mice and the invasion and metastatic ability of the cells was observed in vivo and in vitro.Results and Conclusion The invasion and metastasis activity of 5-8F-H3B-EGFP(an in vivo isolate with enhanced liver metastatic behaviors)were enhanced obviously in comparison with the parent cell line 5-8F-EGFP.This subline may be useful for cloning genes related to liver metastasis of NPC. |
| Keywords: | nasopharyngeal carcinoma liver metastasis invasion subline |
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