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| NF-κB、AP-1及caspase-3在SC58125诱导的HepG2细胞凋亡中的作用 | |
| 引用本文： | 柯小龙 李红良 等. NF-κB、AP-1及caspase-3在SC58125诱导的HepG2细胞凋亡中的作用[J]. 中国病理生理杂志, 2001, 17(12): 1188-1192 |
| 作者姓名： | 柯小龙 李红良 等 |
| 作者单位： | 广东医学院第一附属医院普外科，广东 湛江 524001；暨南大学医学院药理教研室，广东 广州 510632；中山医科大学第一附属医院心内科，广东 广州 510089；暨南大学医学院病理教研室，广东 广州 510632 |
| 基金项目： | 国家自然科学基金资助(No.39500056) |
| 摘 要： | 目的：探讨SC58125诱导HepG2细胞凋亡的分子机理。方法：应用细胞培养、酶联免疫吸附实验、流式细胞术、RT-PCR、Westernblot及电泳迁移率实验等方法研究SC58125诱导HepG2细胞凋亡及其分子机理。结果：SC58125剂量依赖性地诱导HepG2细胞凋亡，并伴有NF-κB结合活性的抑制、caspase-3的激活、bcl-2 mRNA水平的降低及p53 mRNA的上调，而AP-1的结合活性则没有明显的改变。结论：SC58125诱导HepG2细胞的凋亡，这种效应可能与抑制NF-κB结合活性、激活caspase-3、降低bcl-2 mRNA的表达及上调p53 mRNA的水平有关。 |
| 关 键 词： | 消炎药 非甾类 凋亡 NF-κB 肝肿瘤 |
| 文章编号： | 1000-4718(2001)12-1188-05 |
| 收稿时间： | 2001-09-12 |
| 修稿时间： | 2001-09-12 |
Roles of NF- |
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| KE Xiao-long,LI Hong-liang,CHEN Dan-dan,REN Xian-da,ZHANG Hai-wei,CHEN Xiao-dong,LIU Jian-jun. Roles of NF-[J]. Chinese Journal of Pathophysiology, 2001, 17(12): 1188-1192 | |
| Authors: | KE Xiao-long LI Hong-liang CHEN Dan-dan REN Xian-da ZHANG Hai-wei CHEN Xiao-dong LIU Jian-jun |
| Affiliation: | Department of General Surgery, First Afficiated Hospital Guangdong Medical College, Zhanjiang 524001, China; Department of Pharmacology, Medical College of Jinan University, Guangzhou 510632, China; Department of Cardiology, First Afficiated Hospital, Sun-Yet sen University of Medical Sciences, Guangzhou 510089, China; Department of Pathology, Medical College of Jinan University, Guangzhou 510632, China |
| Abstract: | AIM: To explore the molecular mechanisms of SC58125 on apoptosis in HepG2 cells. METHODS: Cell culture, ELISA, flow cytometry, RT-PCR, Western blot and electrophoretic mobility shift assay (EMSA) analysis were employed to clarify the effect of SC58125 on apoptosis in HepG2 cells and related molecular mechanisms. RESULTS: SC58125 induced apoptosis in HepG2 cells in a concentration-dependent manner, which was accompanied by inhibition of NF-κB, activation of caspase-3, decrease of bcl-2 mRNA and increase of p53mRNA. However, no significant changes were found in the DNA binding of AP-1. CONCLUSION: SC58125 induces apoptosis in HepG2 cells, which may be related to the inhibition of NF-κB, activation of caspase-3, decrease of bcl-2 mRNA and increase of p53mRNA. |
| Keywords: | Anti-inflammatory agents non-steroidal Apoptosis NF-kappa B Liver neoplasms |
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