虫草素联合谷氨酰胺对LPS诱导的脓毒症大鼠炎症失衡及肝肺病理变化的影响

目的　研究虫草素（cordycepin，Cop）联合谷氨酰胺（glutamine，Gln）对脂多糖（lipopolysaccharides，LPS）诱导的脓毒症大鼠炎症失衡和肝肺病理变化的影响及其可能机制。 方法　将大鼠按体重随机分为5组：对照组、模型组（LPS组）、LPS+Cop组、LPS+Gln组和LPS+Cop+Gln组，腹腔注射LPS（5 mg/kg）诱导建立脓毒症大鼠模型。ELISA检测外周血中炎症因子IL-6、TNF-α、IL-1β和IL-10的含量；HE染色观察肝肺组织的病理损伤情况；TUNEL染色观察肝肺组织的细胞凋亡情况；Western blot检测肝肺组织中Caspase-3表达水平及NF-κB p65的磷酸化情况 。 结果　LPS+Cop组、LPS+Gln组和LPS+Cop+Gln组均能逆转LPS诱导的促炎因子（IL-6、TNF-α、IL-1β）的高表达和抗炎因子（IL-10）的低表达（P<0.05），减轻病理损伤，抑制细胞凋亡（P<0.05），降低凋亡蛋白Caspase-3高表达（P<0.05），下调NF-κB p65的磷酸化水平（P<0.05）。 结论　在LPS诱导的脓毒症大鼠模型中，虫草素联合谷氨酰胺能有效改善其炎症失衡和肝肺病理变化。

Effects on inflammatory imbalance and pathological changes of liver and lung of cordycepin combined with glutamine in LPS-induced sepsis rats

Objective To study the effects on inflammatory imbalance and pathological changes of liver and lung of cordycepin (Cop) combined with glutamine (Gln) in sepsis rats induced by lipopolysaccharide (LPS) and its possible mechanism. Methods Rats were randomly divided into five groups according to body weight: a control group, a model group (LPS group), a LPS+Cop group, a LPS+Gln group and a LPS+Cop+Gln group. The sepsis rat model was established by intraperitoneal injection of LPS (5 mg/kg). The levels of inflammatory factors IL-6, TNF-α, IL-1β and IL-10 in peripheral blood were detected by ELISA. The pathological damage of liver and lung tissue were observed by HE staining. The apoptosis rate of liver and lung tissue were detected by TUNEL staining. The expression of Caspase-3 and the phosphorylation of NF-κB p65 in liver and lung tissue were detected by Western blot. Results In the LPS+Cop group, LPS+Gln group and LPS+Cop+Gln group, reversal of high expressions of LPS-induced pro-inflammatory factors (IL-6, TNF-α, IL-1β) and low expression of anti-inflammatory factors (IL-10) could be observed (P<0.05). Also, alleviation of pathological damage, inhibition of apoptosis (P<0.05), reduction of high expression of apoptotic protein Caspase-3 (P<0.05) and down-regulation the phosphorylation level of NF-κB p65 (P<0.05) were observed. Conclusions Cordycepin combined with glutamine can effectively improve inflammation imbalance and pathological changes of liver and lung in LPS-induced sepsis rats.