白花蛇舌草提取物体外对人胃癌细胞SGC-7901增殖抑制作用的实验研究

目的探讨白花蛇舌草提取物体外对人胃癌细胞SGC-7901增殖的抑制作用。方法通过体外无菌传代培养人胃癌细胞SGC-7901，将白花蛇舌草提取物以0．05mg/mL、0．1mg／mL、0．15mg／mL、0．2mg／mL四个不同浓度作用于该细胞，24h后采用MTF法检测细胞活性及数量。结果药物组0．05mg／mL和0．1mg／mL浓度孔的吸光值与对照组相比无显著性差异（P〉0．05），而0．15mg／mL和0．2mg／mL浓度孔的吸光值明显小于对照组（P〈0．05）。结论白花蛇舌草提取物体外对人胃癌细胞SGC-7901增殖有一定的抑制作用，且抑制作用的强弱可能跟药物浓度有关，值得进一步研究。

Experimental Research of Hedyotic Diffusa Extract on the Inhibition of the Proliferation of Human Gastric Cancer Cell Line SGC-7901 in Vitro

Objective To investigate the inhibition of hedyotic diffusa extract on the proliferation of human gastric cancer cell line SGC - 7901 in vitro. Methods The human gastric cancer cell line SGC - 7901 was subcuhivated aseptically in vitro. Hedyotic diffusa extract acted on such cell at four different concentrations ,0.05 mg/mL,0.1 mg/mL,0.15 mg/mL and 0.2 mg/mL successively. 24 h later, MTY assay was adopted to determine the activity and numbers of cells. Results The absorbance in drug groups of 0.05 mg/ mL and 0.1 mg/mL concentration holes had no apparent difference compared with that in control group（ P 〉 0.05） ,that at 0.15 mg/mL and 0.2 mg/mL concentration holes was significantly lower than that in control group （ P 〈 0.05）. Conclusion Hedyotie diffusa extract has a certain inhibition in vitro on the proliferation of human gastric cancer cell line SGC -7901. Moreover, the strength of its inhibition is relevant probably with drug concentration,which deserves a further research.