发育期补充DHA对大鼠学习记忆的影响

目的探讨发育期补充DHA对大鼠学习记忆的影响.方法以出生前后脑发育关键期大鼠为研究对象,设一个对照组和添加DHA的3个剂量组(添加DHA的量分别为0、75、15、30g/kg饲料),喂养期限为妊娠第一日至仔鼠45日龄.选用跳台法和避暗法,进行幼鼠学习行为测试,并同步测定幼鼠海马内单胺类神经递质5-HTT、NE、DA和肽类神经递质生长抑素(SS)的含量.结果与对照组相比,15g/kg、30g/kg饲料组幼鼠跳台潜伏期延长,错误次数减少,避暗潜伏期延长,且15g/kg组最显着(P<0.05);补充DHA的各剂量组海马内5-HT、DA、SS含量均显着高于对照组(P<0.05).结论(1)发育期补充一定剂量的DHA,可增强大鼠学习记忆能力,较适剂量为15g/kg饲料;(2)DEIA增强学习记忆可能与海马内5-HT、DA、SS量增高有关,且存在其它递质或递质外系统的复杂调控.

Effect of DHASupplmentation during Development on Rats Learning and Memory Ability.

This study was performed to determine the effects of dietary supplementation of fish oil rich in DHA during development of rats on the learning and memory ability of the young rats.A control group(O)of maternal rats were fed with fundamental diet and their young rats received similar diet from weanling to the age of 45 days.Other three groups were fed with fundamental diet fish oil(doses of DHA were 7.5(A),15(B),30(C)g/kg diet).The findings showed that learning ability of group B and C improved compared to the control group(P<0 05) and grou B was the most significant;5 HT,DA and SS contents of young rats hippocampus were higher in group A,B and C compared to the control group.Conclusion:1.Supplementation of DHA during development could improve learning ability of young rats and the optimal dose was 15g/kg diet.2.DHA improving learning ability might related to 5 HT,DA,SS rising in hippocampus and other transmitter or other michanism.