SATB1在胃癌细胞SGC-7901中表达的研究

目的 通过检测胃癌细胞株SGC-7901和胃黏膜上皮细胞GES-1中SATB1 mRNA和蛋白的表达水平,探讨其在胃癌发展和转移中的作用.方法 利用荧光定量PCR、RT-PCR检测胃癌细胞株SGC-7901和胃黏膜上皮细胞GES-1中SATB1 mRNA的转录水平表达,通过免疫荧光染色检测SATB1在两种细胞中的表达差异.结果 SATB1 mRNA在SGC-7901中的表达程度显著高于GES-1细胞,免疫荧光染色显示SATB1在胃癌细胞株的胞浆和胞核内均有分布,呈强阳性染色,而在GES-1细胞中无表达.结论 SATB1在胃癌细胞株SGC-7901中mRNA和蛋白水平均呈现高表达,提示SATB1的表达水平可能与胃癌发生发展密切相关,有望成为判断胃癌预后的一个指标.

Expression of SATB1 in gastric cancer cell line SGC-7901

Objective To detect the expression of special AT-rich sequence-binding protein-1 （ SATB 1 ） mRNA and pro- tein in gastric cancer cell line SGC-7901 and gastric epithelial cell GES-I, and explore the role of SATBI in the develop- ment of gastric cancer. Methods SATB1 expression was detected using real-time fluorescence quantitative PCR, RT-PCR and immunofluorescence in gastric epithelial cell GES-1 and gastric cancer cell SGC-7901. Results In comparison with GES-1 cells,SGC-7901 cells showed overexpression of SATB1 mRNA. SATB1 was found to localize in the cytoplasm and cell nuclei of SGC-7901 cells, and showed a strong positive staining for SATB1 in immunofluorescence assay. Conclusion SATB1 can highly express in gastric cancer cell,which might be associated with the development of gastric cancer and may potentially be used as all indicator for predicting the prognosis of gastric cancer.