| Mash1 efficiently reprograms rat astrocytes into neurons简 |
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| 引用本文： | Daofang Ding,Leqin Xu,Hao Xu,Xiaofeng Li,Qianqian Liang,Yongjian Zhao,Yongjun Wang. Mash1 efficiently reprograms rat astrocytes into neurons简[J]. 中国神经再生研究, 2014, 9(1): 25-32. DOI: 10.4103/1673-5374.125326 |
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| 作者姓名： | Daofang Ding Leqin Xu Hao Xu Xiaofeng Li Qianqian Liang Yongjian Zhao Yongjun Wang |
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| 基金项目： | This study was supported by the National Basic Research Program of China （973 Pro- gram）, No. 2010CB530400; the Key Project of National Natural Science Foundation of China, No. 30930111. |
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| 摘 要： | To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mashl and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mashl was not detectable. Thus, we hypothesized that Mashl alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mashl. One week later, we observed the changes in morphology of astrocytes, which showed typical neuro- nal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mashl than in control cells. These results indicate that Mashl alone can reprogram astrocytes into neurons.
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| 关 键 词： | 星形胶质细胞 SD大鼠 神经元 编程效率 细胞表达 成纤维细胞 重编程 神经细胞 |
Mash l efficiently reprograms rat astrocytes into neurons |
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| Daofang Ding,Leqin Xu,Hao Xu,Xiaofeng Li,Qianqian Liang,Yongjian Zhao,Yongjun Wang. Mash l efficiently reprograms rat astrocytes into neurons[J]. Neural Regeneration Research, 2014, 9(1): 25-32. DOI: 10.4103/1673-5374.125326 |
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| Authors: | Daofang Ding Leqin Xu Hao Xu Xiaofeng Li Qianqian Liang Yongjian Zhao Yongjun Wang |
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| Affiliation: | [1]Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, China [2]Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China |
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| Abstract: | To date, it remains poorly understood whether astrocytes can be easily reprogrammed into neurons. Mash1 and Brn2 have been previously shown to cooperate to reprogram fibroblasts into neurons. In this study, we examined astrocytes from 2-month-old Sprague-Dawley rats, and found that Brn2 was expressed, but Mash1 was not detectable. Thus, we hypothesized that Mash1 alone could be used to reprogram astrocytes into neurons. We transfected a recombinant MSCV-MASH1 plasmid into astrocytes for 72 hours, and saw that all cells expressed Mash1. One week later, we observed the changes in morphology of astrocytes, which showed typical neuronal characteristics. Moreover, β-tubulin expression levels were significantly higher in astrocytes expressing Mash1 than in control cells. These results indicate that Mash1 alone can reprogram astrocytes into neurons. |
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| Keywords: | nerve regeneration Mash-l Brn2 β-tubulin reprogram plasmid retrovirus astrocytes neurons the National Basic Research Program of China （973 Program） neural regeneration |
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