| NIPAB法测定青霉素G酰化酶酶活力的分析和改进 |
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| 引用本文： | 施晓疌,王永红,张嗣良,储炬,庄英萍. NIPAB法测定青霉素G酰化酶酶活力的分析和改进[J]. 中国医药工业杂志, 2008, 39(9) |
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| 作者姓名： | 施晓疌 王永红 张嗣良 储炬 庄英萍 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心,上海,200237 |
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| 摘 要： | 原NIPAB(2-硝基-5-苯乙酰胺基苯甲酸)法以乙醇为终止剂,采用该法测定发酵制得的青霉素G酰化酶酶活力时,常遇到终止反应不完全、杂质等非酶反应因素显著影响测定结果的问题,因此必须对发酵样品进行预处理。本研究基于反应产物吸光度变化速率,改进为动态方法测定酶活力,不需预处理和终止剂,提高了精确度并简化了操作。
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| 关 键 词： | 青霉素G酰化酶 NIPAB 动态法 改进 |
Evaluation and Improvement of Determination of Penicillin G Acylase Activity using NIPAB Method |
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| SHI Xiao-jie,WANG Yong-hong,ZHANG Si-liang,CHU Ju,ZHUANG Ying-ping. Evaluation and Improvement of Determination of Penicillin G Acylase Activity using NIPAB Method[J]. , 2008, 39(9) |
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| Authors: | SHI Xiao-jie WANG Yong-hong ZHANG Si-liang CHU Ju ZHUANG Ying-ping |
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| Abstract: | Ethanol was usually used as the terminator of NIPAB(2-nitro-5-phenylacetamidobenzoic acid) method for the determination of penicillin G acylase activity.It was found that it couldn't stop the reaction thoroughly and the non-enzymatic interferences affected the result obviously so that the pretreatment of sample was essential.A kinetic method based on the absorbance-change rate of the product was established,in which sample pretreatment could be omitted resulting in the simplification of the process and the improvement of the accuracy of determination. |
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| Keywords: | penicillin G acylase NIPAB kinetic method improvement |
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