基质金属蛋白酶-3与细胞外基质金属蛋白酶诱导因子在乳腺癌演变过程中的表达及意义

目的:检测基质金属蛋白酶-3(MMP-3)和细胞外基质金属蛋白酶诱导因子(EMMPRIN)在乳腺癌演变过程中的表达并探讨其意义。方法:应用免疫组化二步法,检测58例乳腺癌、28例非典型导管增生、40例普通导管增生及20例正常乳腺组织中MMP-3和EMMPRIN蛋白的表达,并采用原位杂交法检测各种病变及正常乳腺组织各20例中MMP-3mRNA、EMMPRIN mRNA的表达。结果:MMP-3、EMMPRIN及MMP-3mRNA、EMMPRIN mRNA在乳腺癌组织(16.98±1.47,20.42±1.54,12.88±1.34,12.30±1.25)和非典型导管增生组织(19.05±1.38,17.38±1.69,13.18±1.34,13.49±1.25)中的IOD值较普通导管增生组织(10.96±1.20,10.23±1.09,10.47±1.12,10.23±1.09)及正常乳腺组织(10.36±1.05,10.22±1.20,10.47±1.14,10.21±1.06)高(P<0.01)。MMP-3、EMMPRIN和MMP-3 mRNA、EMMPRIN mRNA在乳腺癌和非典型导管增生组织中的表达水平...

Expressions of matrix metalloproteinase-3 and extracellular matrix metallo-proteinase inducer in the carcinogenesis of breast cancer and theirs significances

Objective To explore the expressions of matrix metalloproteinase-3(MMP-3) and extracellular matrix metallo-proteinase inducer(EMMPRIN) in the carcinogenesis of breast cancer and their significance. Methods The expressions of MMP-3 and EMMPRIN proteins were detected in 58 breast cancers, 28 atypical hyperplasia, 40 ductule hyperplasia, and 20 normal breast tissues using immunohistochemical method, so were the expressions of MMP-3 mRNA and EMMPRIN mRNA in 20 of each type of tissues using in situ hybridization...