神经调节蛋白-1与碱性成纤维细胞生长因子对大鼠神经干细胞增殖和分化的影响

目的:探讨神经调节蛋白-1(NRG-1)和碱性成纤维细胞生长因子(bFGF)对神经干细胞(NSCs)增殖和分化的影响.方法:从新生大鼠大脑组织分离NSCs,进行体外培养,分别用bFGF、 NRG-1和NRG-1加bFGF处理,观察各组神经球的形成情况;应用免疫细胞化学法鉴定NSCs及检测分化后神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)、 2,3-环核苷酸磷酸二酯酶(CNP)的表达.结果:NRG+bFGF组和bFGF组形成的神经球数量和直径的差别不明显,但都明显多于和大于NRG组和对照组.免疫细胞化学显色结果显示,NRG+bFGF组、bFGF组中的神经球分化出的NSE、 GFAP、 CNP阳性细胞数量明显多于NRG组和对照组,尤其是NRG+bFGF组分化的NSE、CNP阳性细胞的突起较bFGF组更长和增多.结论:NRG-1与bFGF合用能促进NSCs的增殖和分化,促进分化的神经元、少突胶质细胞突起的生长.

Effects of neuregulin-1 and bFGF on proliferation and differentiation of neural stem cells from postnatal rat

Objective:To investigate the effects of neuregulin-1 (NRG-1) and basic fibroblast growth factor (bFGF) on proliferation and differentiation of cultared neural stem cells. Methods:The neural stem cells were isolated from postnatal rat telencephalon, and then treated with bFGF, NRG-1 and NRG-1 plus bFGF. The formation of neurospheres in the corresponding group was carefully monitored. Either the proliferation of neural stem cells or their differentiation into new neural and glial cell types could be identified by immunocytochemistry. Results:The number and diameter of the neurospheres showed no distinct difference between the NRG+bFGF group and bFGF group. However, more and larger neurospheres were observed in the NRG+bFGF group and bFGF group than those in the NRG group and control group. Furthermore, the immunocytochemical results showed that the neurospheres in the NRG+ bFGF group and bFGF group could differentiate into more cell types, such as antigen of neurons, astrocytes and oligodendrocytes, than those in the NRG group and control group. In particular, the protuberances of the NSE or CNP positive cells in the NRG+bFGF group were much longer and more than those in the bFGF groups. Conclusion:The experimental results indicate that the combination of NRG-1 and bFGF can promote the proliferation and differentiation of the neural stem cells, which are isolated from postnatal rat telencephalon, as well as the growth of the protuberances of neurons and oligodendrocytes.