一氧化氮合成酶在痉挛脑血管壁动态变化的实验研究

 目的研究蛛网膜下腔出血(SAH)后痉挛的血管壁中内皮型一氧化氮合成酶(eNOS)的演变过程.方法采用兔SAH模型,全部实验动物于首次注血前和处死前行脑血管造影,取基底动脉进行免疫组织化学分析,了解eNOS的免疫表达.结果脑血管造影显示第3、5、7和10 d组注血后基底动脉直径明显减小(P＜0.05),而对照组和第14 d组减小不明显(P＞0.05).免疫组化结果表明,对照组见有eNOS重度染色,14 d组染色与对照组接近,第3、5、7、10 d组染色较对照组色淡,以7 d组最淡.结论SAH后痉挛期血管壁中eNOS减少,进一步证明了一氧化氮减少在SAH后脑血管痉挛(CVS)发展过程中发挥了重要作用.

Nitric oxide synthase level in rabbit basilar artery after subarachnoid hemorrhage

Objective To investigate the levels of endothelial nitric oxide synthase(eNOS) following subarachnoid hemorrhage in rabbit basilar arteries.Methods 36 rabbits were assigned to six groups:group 1 control;group 2,sacrificed 3 days after the first intracisternal injection of blood; groups 3,4,5 and 6, given twice intra cistemal injection of blood and sacrificed 5 ,7,10, and 14 days after the first injection, respectively. Constriction of the basilar artery was confirmed by arterial angiography. Rabbit basilar arteries were analyzed by means of im-munochistochemistry for eNOS.Results In groups 2,3,4 and 5,angiography showed the basilar artery decreased in diameter, with significant differences between the two angiograms ( P < 0.05), while in groups 1 and 6, the basilar arteries decreased in diameter, but the differences were not significant between the two angiograms( P > 0.05) . On immunohistochemical study,highly positive staining was found in basilar arteries of group 1; immunohistochemical staining in group 6 was similar to that in groupl; group 2,3,4 and 5, especially group 4, showed slightly immunohistochemical staining. Conclusions Endothelial NOS decreases in rabbit basilar arteries after subarachnoid hemorrhage, the investigation indirectly proves that NO is a major pathogenic factor of cerebral vasospasm.