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Regulative effect of P38MAPK on release of TNF
Authors:LU Jiakai  YANG Zhihuan  JIANG Jianxin  WANG Zhengguo  ZHU Peifang
Affiliation:Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China
Abstract:
Objective: To evaluate activation of P38 mitogen-activatedprotein kinase (P38MAPK) in alveolar macrophage (AM), release of TNFα and NO from cells, and their relationship following lipopolysacchride (LPS) stimulation.Methods: AM was isolated from branch alveolar lavage fluid (BALF). The activation of P38MAPK was assayed by Westernblot. SB203580, a specific inhibitor of P38MAPK, was used with gradient concentration to evaluate the regulative effect of P38MAPK on the release of TNFα and NO from AM.Results: P38MAPK was activated by LPS (100 ng/ml) with peak activation at 30 minutes. The activation of P38MAPK was inhibited by SB203580. The secretion of TNFα and NO stimulated with LPS increased (P<0.01) and was inhibited by SB203580 significantly.Conclusions: The results indicate that P38MAPK is involved in the secreting process of TNFα and NO following LPS stimulation. P38MAPK may be an important site for controlling the secretion of both inflammatory mediators during lung inflammatory disorders.
Keywords:Macrophages,alveolar  Tumor necrosis factor  Nitric oxide  Lipopolysaccharides  P38MAPK
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