Livin蛋白和LivinmRNA在散发性大肠管状腺瘤癌变过程中的表达

目的探讨凋亡抑制蛋白Livin和LivinmRNA在散发性大肠管状腺瘤癌变过程中的可能作用。方法采用免疫组化和原位杂交方法检测87例散发性大肠管状腺瘤伴上皮不同程度异型增生及癌变组织中Livin在蛋白和mR-NA水平的表达情况。结果 Livin蛋白在正常大肠黏膜、大肠管状腺瘤伴不同异型增生及腺瘤癌变病例中的阳性表达率分别为14.3%、52.3%和77.3%,腺瘤伴异型增生组和癌变组中Livin蛋白的表达明显高于正常大肠黏膜组（P〈0.05）,且癌变组中Livin蛋白的表达高于腺瘤伴异型增生组（P〈0.05）;Livin蛋白的表达随腺瘤异型增生程度的增高而增高,但无统计学意义（P〉0.05）。大肠管状腺瘤伴异型增生组和癌变组中Livin在mRNA水平的阳性表达率分别为50.8%和81.8%,均明显高于正常大肠黏膜组,而且癌变组中LivinmRNA的表达明显高于腺瘤伴异型增生组（P〈0.05）;LivinmRNA随腺瘤异型增生程度的增高呈递增趋势,但无统计学意义（P〉0.05）。Livin在蛋白和mRNA水平的表达存在一致性（P〈0.05）。结论 Livin在大肠管状腺瘤癌变过程中可能起一定作用。

Expression of Livin and Livin mRNA in the carcinogenesis of sporadic colorectal tubular adenoma

Objective To explore the possible role of Livin in the carcinogenesis of sporadic colorectal tubular adenoma.Methods The immunohistochemical staining was used to detect the expression of Livin at protein level in 87 sporadic colorectal tubular adenomas with different dysplasia and with cancerous changes.The expression of Livin at mRNA level was studied in above cases by in situ hybridization method as well.Results The positive expression rate of Livin was 14.3%,52.3%,77.3% respectively in normal colorectal ...