酒精性股骨头缺血性坏死动物模型的建立与探讨

目的探讨适合于酒精性股骨头缺血坏死的动物模型。方法雄性家兔 60只 ,随机分为 2组 ,采用灌胃法 ,实验组给予含乙醇 50 %的烈性白酒 8mL (kg .d) ,对照组给予等体积的生理盐水。于 1、2、3、6个月分批处死取股骨头观察组织学变化。结果实验组家兔骨髓内造血组织减少 ,脂肪细胞增大 ;骨小梁变细、稀疏 ,骨细胞脂肪变性 ,空骨陷窝增多。透射电镜下见大量脂质沉积、坏死 ;胶原纤维稀疏、排列紊乱、部分断裂。结论雄性家兔早期酒精性股骨头缺血坏死组织学变化与人类相似 ,适合用于制作酒精性股骨头缺血坏死的模型。

Establishment on animal model of alcohol-induced avascular necrosis of the femoral head

objective To explore and establishment reasonable animal model of alcoholinduced avascular necrosis of the femoral head. Methods 60 male rabbits were divided into two groups randomly. Experimental group were administered the wine containing 50% of alcohol at dosage of 8ml/(kg.d) and isodose saline as controls by pouring into stomach with a plastic tube. The rabbits were sacrificed and studied the histological change of the femoral head in batches 1,2,3,6 months after the start of the procedures. Results The hematopoietic tissue of the femoral head marrow in experimental group were decreased and the fatty tissue were increased , the trabeculace became thinner and sparse, Afat degeneration was seen inosteocytes with the empty osteocytes lacuna in the trabeculace being elevated. The transmissible electron microscope study of the osteocyte showed lipid deposition and necrosis, collagenfibers revealed rarefaction and its arrangement deranged with portion breaking. Conclusion The change of in early histology of alcohol-induced avasoularnecrosis of the femoral head in male rabbit similar homo sapiens. Rabbit can be used animal model as alcohol-induced avascular necrosis of the femoral head.