| FGA等15个短串联重复序列在融水县苗族群体中的遗传学分析 |
| |
| 引用本文： | 周丽宁,覃耀春,徐林,李松峰,邓琼英,龚继春.FGA等15个短串联重复序列在融水县苗族群体中的遗传学分析[J].解剖学报,2008,39(5):683-687. |
| |
| 作者姓名： | 周丽宁 覃耀春 徐林 李松峰 邓琼英 龚继春 |
| |
| 作者单位： | 1.广西医科大学解剖学教研室人类学研究室,南宁 530021; 2.广西医科大学公共卫生学院,南宁 530021 |
| |
| 摘 要： | 目的 了解广西融水县苗族群体中15个短串联重复序列(STR):TPOX、TH01、CSFIPO、D19S433、vWA、D18S51、D5S818、FGA、D8S1179、D21S11、D7S820、D3S1358、D13s317、D16S539、D2S1338遗传多态的分布情况.方法 用枸橼酸钠抗凝法采集融水县苗族的血样,酚.氯仿抽提法提取DNA,用AmpFISTRR Identifiler TM PCR Amplification kit荧光标记复合扩增技术和ABI 3100型遗传分析仪对DNA进行扩增检测.结果 15个STR分别检测出5～20种等位基因,11～62种基因型,基因型的分布符合Hardy-Weinberg平衡定律,等位基因频率和基因型频率分布在0.002 4～0.4663和0.004 8～0.274 0之间;平均杂合度0.781 1,累积个人识别力1.000 000 000 00,累积非父排除率0.999 999 998,多态信息总量0.999 999 999 4.结论 广西融水苗族群体的15个短串联重复序列基因座具有高度多态性和遗传稳定性,实用价值高,可作为广西苗族群体的民族遗传学研究和法医学鉴定的基础数据.
|
| 关 键 词： | 短串联重复序列 等位基因 基因频率 酚-氯仿抽提法 苗族 |
| 收稿时间： | 2007-5-24 |
| 修稿时间： | 2007-7-6 |
STUDY ON POLYMORPHISMS OF 15 STR LOCI IN MIAO ETHNIC GROUP OF RONGSHUI COUNTY IN GUANGXI PROVINCE |
| |
| ZHOU Li-ning,QIN Yao-chun,XU Lin,LI Song-feng,DENG Qiong-ying,GONG Ji-chun.STUDY ON POLYMORPHISMS OF 15 STR LOCI IN MIAO ETHNIC GROUP OF RONGSHUI COUNTY IN GUANGXI PROVINCE[J].Acta Anatomica Sinica,2008,39(5):683-687. |
| |
| Authors: | ZHOU Li-ning QIN Yao-chun XU Lin LI Song-feng DENG Qiong-ying GONG Ji-chun |
| |
| Institution: | 1.Department of Anatomy, Guangxi Medical University, Nanning 530021, China;2.College of Public Health, Guangxi Medical University, Nanning 530021, China |
| |
| Abstract: | Objective To investigate the distribution patterns of 15 short tandem repeats(STR) loci(TPOX,TH01,CSF1PO,D19S433,vWA,D18S51,D5S818,FGA,D8S1179,D21S11,D7S820,D3S1358,D13S317,D16S539,D2S1338) in Miao ethnic group of Rongshui County in Guangxi Province.Methods The sodium citrated-blood specimens were collected from 208 healthy unrelated Miao individuals(man: 102,female: 106) in Rongshui County in Guangxi Province,and then the DNAs from the samples were extracted by phynol-chloroform technique.The DNAs were amp... |
| |
| Keywords: | Short tandem repeat Allele Gene frequency Phynol-chloroform technique Miao Ethnic |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《解剖学报》浏览原始摘要信息 |
| 点击此处可从《解剖学报》下载免费的PDF全文 |
|
