BAG-1在大肠癌中的表达及其临床意义

目的探讨Bag-1基因在大肠癌组织和正常大肠组织中的表达变化以及与淋巴结转移之间的关系和临床意义。方法联合采用Western blot和RT-PCR方法分别检测经手术切除的55例大肠癌组织标本中Bag-1的表达,另取25例正常大肠粘膜组织为对照组。结果（1）55例大肠癌均检测到不同程度的Bag-1蛋白表达,相对强度为(5～109,64.09±31.59)，Bag-1在有淋巴结转移的大肠癌组织中蛋白相对强度(30.34～109,81.05±19.64)高于无淋巴结转移的大肠癌组织中蛋白相对强度(5～70.13,26.19±16.32)(P<0.001)，但两者均明显高于正常大肠黏膜Bag-1蛋白相对强度(0～10,4.28±2.82)(P<0.001)。（2）55例大肠癌组织中Bag-1mRNA表达量相对值为(0.33～1.21，0.74±0.26)；在有淋巴结转移的大肠癌组织中表达量相对值(0.52～1.21，0.87±0.21)高于无淋巴结转移的大肠癌组织中表达量相对值(0.33～0.65，0.45±0.09)(P<0.001)，但两者也均明显高于正常大肠黏膜组织(0～0.22，0.10±0.06）(P<0.001)。结论Bag-1高表达对大肠癌的发生、发展起重要作用,Bag-1表达水平与大肠癌的淋巴结转移有关,可作为预测大肠癌转移潜能的生物学指标，检测Bag-1表达水平可能有助于患者预后的判断。

Expression and Clinical Significance of BAG-1 in Human Colorectal Carcinomas

Objective To investigate the relationship and clinical significance of Bag-1 expression in colorectal carcinoma tissues and normal colorectal tissues,and its relationship with lymphnode metastasis.Methods The expressions of Bag-1 in 55 patients’ colorectal carcinoma tissues(case group) and 25 normal colorectal tissues(control group) were examined by Western blot analysis and RT-PCR.Results(1) The expressions of Bag-1 in 55 colorectal carcinoma tissues were inordinately detected,the protein relative strength of Bag-1 was(5~109,64.09±31.59).We found that the protein relative strength of Bag-1 in colorectal carcinoma tissues with lymphnode metastasis(30.34~109,81.05±19.64) was higher than that in colorectal carcinoma tissues without lymphnode metastasis(5~70.13,26.19±16.32)(P<0.001),however,both of them were obviously higher than that in normal colorectal tissues(0~10,4.28±2.82)(P<0.001).(2) The gene fragments of Bag-1 and β-actin primers amplified were clearly visible by electrophoresis and EB staining.The length of the gene fragments amplified was 331 bp and 254 bp respectively,which was consistent with that we designed.The relative expression value of Bag-1 mRNA in 55 colorectal carcinoma tissues was(0.33~1.21,0.74±0.26).The relative expression value of Bag-1 mRNA in colorectal carcinomas tissues with lymphnode metastasis(0.52~1.21,0.87±0.21) was higher than that in colorectal carcinoma tissues without lymphnode metastasis(0.33~0.65,0.45±0.09)(P<0.001),however,both of them were significantly higher than that in normal colorectal tissues(0~0.22,0.10±0.06)(P<0.001).Conclusion The overexpression of Bag-1 plays an important role in the development and progression of colorectal carcinomas.The expression level of Bag-1 is correlated with lymphnode metastasis.Bag-1 can serve as a biomarker to prognosticate the metastasis potentiality of colorectal carcinomas.Detecting Bag-1 may contribute to estimate the prognosis of patients.