兔膀胱移行上皮细胞构建组织工程尿道的初步研究

目的 探索以膀胱移行上皮细胞为种子细胞构建组织工程尿道的可行性。方法 取兔膀胱移行上皮细胞，体外培养传代后作为种子细胞与自制胶原膜体外联合培养并行裸鼠体内移植，通过对复合物扫描电镜、HE染色及角蛋白免疫组化检测，了解细胞在材料上的生长情况。结果 2h后移行上皮细胞在胶原膜上贴附生长，复合物移植体内20d后，移行上皮细胞分化成层，层厚1～5层。结论 该方法获取的膀胱移行上皮细胞是构建组织工程尿道的良好种子细胞，与胶原膜构建的复合物在体内形成了尿道类似物，有望成为替代尿道的组织工程材料。

Preliminary study of urethra construction with rabbit bladder transitional cells by tissue engineering

Objective To investigate the feasibility of using transitional cells as seeding cells to construct urethra by tissue engineering. Methods Transitional cells isolated from bladders of New Zealand rabbit were cultured as seeding cells, which were implanted on the collagen membrane for culture in vitro . The cell scaffold complex was transplanted in nude mice. Scanning electron microscopy, HE staining and immunocytochemical staining of cytokeratin were used to evaluate the growth of cells on the collagen membrane. Results Transitional cells adhered to collagen membranes and grew at 2 h after implantation. On day 20 after transplantation in vivo , the transitional cells lined in continuous layers of 1 to 5 cell thickness at some parts of the complex. Conclusion Transitional cells isolated in this way are suitable seeding cells to construct urethra by tissue engineering. The cell scaffold complex can form an analog of normal urethra after transplantation in vivo .