| 辣根过氧化物酶标记的纯视细胞层移植 |
| |
| 引用本文： | 潘峰,王薇,沈丽,杨磊. 辣根过氧化物酶标记的纯视细胞层移植[J]. 解剖学报, 2002, 33(1): 104-107 |
| |
| 作者姓名： | 潘峰 王薇 沈丽 杨磊 |
| |
| 作者单位： | 北京大学第三临床医学院眼科,100083;北京大学基础医学院解剖学系,100083 |
| |
| 基金项目： | 国家自然科学基金资助项目 (3 9870 847),卫生部科学研究基金资助项目 (98 1 2 63 ) |
| |
| 摘 要： | 目的 利用HRP标记Wistar鼠的视细胞 ,观察移植的纯视细胞在受体的分布。 方法 30 %的HRP标记Wistar鼠视细胞 ,经外路途径移入皇家外科学院鼠 (RoyalCollegeofSurgeonRat,RCS)的视网膜下腔 ,术后 2周取RCS鼠眼做冰冻切片 ,组织化学染色法染色 ,普通光学显微镜下观察。 结果 在视细胞移植术后 2周 ,移植视细胞存活 ,HRP标记的Wistar鼠纯视细胞层在RCS鼠视网膜内呈褐色 ,排列平覆。 结论 在RCS鼠视网膜内可以观察到移植的HRP标记的Wistar鼠纯视细胞
|
| 关 键 词： | 辣根过氧化物酶 视细胞移植 大鼠 |
HORSE-RADISH PEROXIDASE HRP-LABELING PHOTORECEPTOR CELL TRANSPLANTATION |
| |
| PAN Feng ,WANG Wei ,SHEN Li ,YANG Lei. HORSE-RADISH PEROXIDASE HRP-LABELING PHOTORECEPTOR CELL TRANSPLANTATION[J]. Acta Anatomica Sinica, 2002, 33(1): 104-107 |
| |
| Authors: | PAN Feng WANG Wei SHEN Li YANG Lei |
| |
| Affiliation: | PAN Feng 1,WANG Wei 1*,SHEN Li 2,YANG Lei 2 |
| |
| Abstract: | Objective We observe the grafted Wistar rat's photoreceptor cell labeled by Horse|radish peroxidase (HRP) in the receptor. Methods The healthy Wistar rat's photoreceptor cells were labeled and grafted into the sub|retinal space of the left eye of the Royal College of Surgeon (RCS) rat by outer|path way. Two weeks later, the eyes of the RCS rats were stained by cyto|chemistry method and processed for light microscopic. Results After two weeks of transplantation, the transplanted photoreceptor cells survived. HRP|labeling photoreceptor cell was stained brown and ranged orderly.Conclusion These results suggest that we can observe the grafted Wistar rat's photoreceptor cell in the RCS rat's retina by transplanting the HRP|labeling photoreceptor cells.;[ |
| |
| Keywords: | Horse|radish peroxides Photoreceptor cell transplantation Rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
