雄性激素抑制感染杜氏利什曼原虫的巨噬细胞的凋亡

目的：研究雄性激素对Ｃ５７ＢＬ／６ｊ雌性小鼠骨髓巨噬细胞凋亡的影响。方法：溴化丙锭染色及透射电镜观察凋亡特征性形态学变化。来自雄性激素和油处理过的小鼠骨髓巨噬细胞分别用杜氏利什曼原虫（Ｌｅｉｓｈｍａｎｉａｄｏｎｏｖａｎｉ）攻击２４ｈ后,检测特征性的ＤＮＡ凋亡梯形。结果：在培养基中去掉Ｍ－ＣＳＦ后可以诱导骨髓巨噬细胞的凋亡。ＤＮＡ片段电泳提示：①在雄性激素和油处理组间凋亡细胞的量没有区别,然而②经杜氏利什曼原虫攻击后,雄性激素处理组的凋亡细胞数明显低于油处理组。结论：雄性激素可以抑制感染了杜氏利什曼原虫的巨噬细胞的凋亡,不感染利什曼原虫时,这种抑制作用并不出现。雄性激素对骨髓巨噬细胞凋亡的抑制作用可能在雄性激素诱导的免疫抑制作用中发挥着重要的作用

TESTOSTERONE INHIBITS APOPTOSIS OF LEISHMANIA DONOVANI INFECTED MACROPHAGES*

AIM: Investigate the effect of the male sex hormone, testosterone (Te), on apoptosis of bone marrow derived macrophages (BMMs) from female C57BL/6j mice METHODS: Propidium iodide (PI) staining and transmission electron microscopy (TEM) techniques were used to investigate apoptosis specific-morphological changes. BMMs derived from Te-treated mice and Oil-treated mice were challenged with Leishmania donovani (L. d.), Oligo-nucleosomal DNA were extracted 24 h post infection to detect apoptosis. RESULTS: The removal of M-CSF from the medium could induce BMM apoptofsis. And the DNA fragmentation assay also indicated that: 1. there was no difference in the amount of apoptotic cells between Te and Oil group; 2. Te + L. d. group had significantly less dead cells than Oil + L. d. group demonstrating that Te could prevent apoptosis of macrophage infected with L. d. to a greater extent. CONCLUSION: Te inhibits apoptosis of the macrophages infected with L. d., however, this inhibition did not occur in the macrophages uninfected with L. d. Te-induced macrophage apoptosis-inhibition may play an important role in Te-induced immunosuppression.