Double wavelength measurement of 3,3',5,5'-tetramethylbenzidine (TMB) provides a three-fold enhancement of the ELISA measuring range |
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| Authors: | S Madersbacher P Berger |
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| Affiliation: | Immunoendocrinology Research Unit, Austrian Academy of Sciences, Innsbruck. |
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| Abstract: | ![]()
A rapid, simple method is described which permits a three-fold enhancement of the working range of ELISA procedures using TMB as a substrate. This consists of measuring absorbance values at a wavelength away from the absorption maximum of TMB and using a predetermined multiplication factor. |
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