电针结合经颅磁刺激对脑缺血大鼠VEGF164 mRNA和CD31表达的影响

目的研究电针结合经颅磁刺激对急性脑缺血大鼠血管生成的影响。方法将55只雄性Wistar大鼠随机分成正常组、模型组、电针组、磁刺激组和电针加磁刺激组。复制急性大脑巾动脉缺血模型，分别施以电针、磁刺激和电针加磁刺激方法处理，采用逆转录-聚合酶链反应、免疫组织化学方法，检测VEGF164mRNA及血小板内皮细胞黏附因子（CD31）的表达。结果电针绢、磁刺激组和电针加磁刺激组梗死灶周同VEGF164mRNA和CD31表达增强（P〈0．05或P〈0．01），尤其以电针加磁刺激组明最。结论电针结合磁刺激可以增强急性脑缺血大鼠梗死灶周围VEGF164mRNA和CD31的表达，从而实现非分子水平的治疗性血管生成作用。

The effects of electroacupuncture combined with transcranial magnetic stimulation on VEGF164 mRNA and CD31 expression in rats with cerebral ischemia

Objective To study the effect of electroacupuncture(EA) combined with transcranial magnetic stimulation(TMS) on the angiogenesis in rats with cerebral ischemia. Methods Fifty-five male Wistar rats were randomly divided into five groups: a normal group, a model group, an EA group, a TMS group and an EA plus TMS group. After the establishment of acute middle cerebral artery occlusion (MCAO), the rats in various groups were accordingly treated with EA, TMS or EA plus TMS, respectively. Then the expression of VEGF164 mRNA and CD31 was observed. Results The expression of VEGF164 mRNA and CD31 around the infarction locus of the three treatment groups increased, especially in the EA plus TMS group. Conclusion EA plus TMS can increase the expression of VEGF164 mRNA and CD31 around the infarction locus and promote the non-molecular angiogenesis in rats with cerebral ischemia.