大鼠局灶性脑缺血再灌注后IL—6水平变化与神经元损害关系的探讨

目的 探讨大鼠局灶脑缺血再灌注后白细胞介素－6（IL－6）水平变化与神经元损害的关系。方法 将60只Wistar大鼠随机分为正常对照组、假手术组、缺血再灌注组。应用线栓法制作大鼠脑缺血再灌注损型，采用免疫组织化学方法检测大鼠脑组织IL－6的变化，用HE染色观察神经细胞损伤与白细胞浸润情况，并与正常对照组及假手术组大鼠比较。结果 IL－6在正常对照组大鼠神经元与胶质细胞仅有少量表达，缺血再灌注6小时时IL－6表达较正常神经元稍增多但无统计学意义（P＞0．05），12小时开始在梗死周围区增高，第3天显著增强，以后逐渐下降。结论 IL－6在缺血性脑损伤中过量表达参与了脑组织的损伤过程。

Investigation of the relationship between IL-6 levels and neuronal damage after cerebral ischemic reperfusion in rats

Objective To investigate the relationship between IL 6 levels and neuronal damage after cerebral ischemic reperfusion in rats.Methods 60 Wister rats were divided randomly into the following groups: normal control group, sham operative group and ischemic reperfusion group. Adopting the model of ischemic reperfusion, the change of IL 6 in the rat's brain tissue was determined with the method of immunohistochemistry, and the neuron necrosis and WBC infiltration were observed by HE dye,as compared with the normal control group and sham operative group.Results There was little expression of IL 6 in neuron and gliocytes of the normal control group,normal neuron could express little Il 6.There was no statistical significance between the expression at 6h reperfusion and that of normal group( P >0.05).The expression began to increase around the infarction at 12h reperfusion,it reached the peak at the 3th day in the boundary zone and then it gradually decreased.Conclusion The overexpression of IL 6 played an important role in the course of cerebral ischemic reperfusion, and could damage the brain tissue.