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| TCRVβ7.1基因修饰T细胞对乳腺癌细胞杀伤作用的研究 | |
| 引用本文： | 胡丽彩,邱曙东,黄树林,刘璋,林月霞,罗利琼. TCRVβ7.1基因修饰T细胞对乳腺癌细胞杀伤作用的研究[J]. 中国免疫学杂志, 2003, 19(2): 97-99,103 |
| 作者姓名： | 胡丽彩 邱曙东 黄树林 刘璋 林月霞 罗利琼 |
| 作者单位： | 1. 西安交通大学医学院,西安,710062 2. 广东药学院生物制药研究所,广州,510224 3. 广州市经济技术开发区医院,广州,510000 |
| 基金项目： | 国家自然科学基金，广东省自然科学基金资助 |
| 摘 要： | 目的：观察TCPVβ7．1基因转染前后正常人外周血淋巴细胞对乳腺癌细胞株杀伤活性的影响。方法：脂质体包裹PdWA3.1vβ7.1后转染健康人PBMC，流式细胞仪检测PdWA3.1Vβ7.1基因表达，改良MIT法检测TCRVβ7．1基因转染前后正常人外周血淋巴细胞对乳腺癌细胞株杀伤活性。结果：TCRVβ7．1基因转染可显著增加正常人PBMC该基因表达，转染前后正常人外周血淋巴细胞对乳腺癌细胞株杀伤活性有显著性差异。结论：用TCR基因修饰可明显提高正常人PBMC对乳腺癌细胞杀伤作用。 |
| 关 键 词： | 乳腺癌 TCRVβ7．1基因 外周血淋巴细胞 流式细胞仪 基因表达 基因修饰 基因转染 |
| 文章编号： | 1000-484X(2003)02-0097-04 |
The experimental study of the cytotoxicity to breast cancer cell line using modified T lymphocyte by TCRVβ7.1 gene |
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| HU Li- Cai,QIU Shu-Dong,HUANG Shu-Lin et al. Medical Colkge of Xi' an University,Xi' an ,China. The experimental study of the cytotoxicity to breast cancer cell line using modified T lymphocyte by TCRVβ7.1 gene[J]. Chinese Journal of Immunology, 2003, 19(2): 97-99,103 | |
| Authors: | HU Li- Cai QIU Shu-Dong HUANG Shu-Lin et al. Medical Colkge of Xi' an University Xi' an China |
| Affiliation: | HU Li- Cai,QIU Shu-Dong,HUANG Shu-Lin et al. Medical Colkge of Xi' an University,Xi' an 710062,China |
| Abstract: | Objective: To investigate the influence of transfection of TCRVJ37.1 gene in cytotoxicity of PBMC of healthy donor to breast cancer cell line MCF-7/S. Methods :pcDN*31vp'1 packaged by lipofectamine were transfected to healthy donor's PBMC,Flow Cytometer Analysis and MTT Colorimetric Assay were used respectively to test the expression of pcDNA3.1VB7.1 gene and the cytotoxicity of PBMC before and after gene transfection of healthy donor to breast cancer cell line MCF-7/S. Results: The expression of TCRVJ37.1 gene after transfection was obviously higher.There was distinguish differences of cytotoxicity before and after gene transfection. Conclusion:The modified healthy donor's PBMC by TCR gene could have stronger cytotoxicity to breast cancer cell line. |
| Keywords: | T cell receptor Gene transfection Breast cancer cell linejCytotoxicity |
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