聚合酶链反应检测乙型肝炎患者心肌组织中HBV DNA

应用巢式聚合酶链反应（ＰＣＲ）技术检测了１８例乙型肝炎（乙肝）患者石蜡包埋心、肝组织中的ＨＢＶ ＤＮＡ，并与其免疫组化及原位杂交结果作了比较。二组引物检测的结果表明：在肝组织中均有ＨＢＶ感染，其中５例有ＨＢＶ复制。心肌组织中ＨＢＶ ＤＮＡ的检出率为５５．６％，不存在ＨＢＶ的复制。心脏病理检查可见一些非特异性改变，如脂变、水肿和纤维素样坏死等。病理变化与心电图、临床症状、体征及ＰＣＲ检测结果无相关性

DETECTION OF HBV AND IN HEART BY PCR

ormalin-fixed paraffin-embedded heart and liver tissues from eighteen autopsy pa-ients with hepatitis B virus(HBV)infection were studied by nested polymerase chainreaction(PCR)for detection of HBV DNA with two groups of primer.The DNA seq-uence represented HBV infection was detected in all liver tissues(100%)and in ten ofheart tissues(55.6%),respectively.The DNA sequence represented HBV replication wasdetected in 5 cases of liver tissues only.The findings of PCR was corresponded with resultof immunohistochemistry and in situ hybridization.The result revealed that HBV couldinfect heart but do not replicate in it.We also found some of the non specific pathologi-cal changes such as fatty degeneration,edema,fibrinoid necrosis and there was no corre-lation between pathological changes,electrocardiogram,clinical findings and result ofPCR.All these findings may suggest that some of the histopathological changes of he-art in the patient with hepatitis B infection may be caused by the immuno-mediatedinjury of immune complex deposited in heart.