丹参素、丹酚酸B与不同血浆蛋白结合率的测定

目的:建立测定丹酚酸B和丹参素与大鼠、牛血清白蛋白(BSA)、人血浆蛋白结合率的方法,比较丹酚酸B和丹参与不同血浆蛋白结合率的差异。方法:采用UPLC测定丹酚酸B和丹参素含量,流动相乙腈(A)-1%甲酸溶液(B)梯度洗脱(0~2 min,18%A;2~3 min,18%~30%A;3~6 min,30%A),检测波长280 nm。通过平衡透析法考察丹酚酸B和丹参素与不同血浆的蛋白结合率。结果:丹酚酸B和丹参素在3种不同血浆中线性范围均为2~200 mg·L-1,透析外液均为0.5~20 mg·L-1。丹酚酸B在大鼠、人血浆和BSA中的平均血浆蛋白结合率分别为90.60%,92.25%,87.55%;丹参素在大鼠、人血浆及BSA中的平均血浆蛋白结合率分别为35.44%,37.71%,33.49%。结论:不同质量浓度的丹酚酸B和丹参素在同一种血浆中的蛋白结合率无显著性差异,丹酚酸B与3种血浆蛋白结合率较丹参素高。

Determination of Protein Binding Rates of Salvianolic Acid B and Danshensu with Different Plasmas

Objective: By establishing UPLC method for determining binding rate between salvianolic acid B,danshensu and rat,bovine serum albumin(BSA),human plasma proteins,to analysis difference of plasma protein binding rate of these two components. Method: UPLC method was employed to determine contents of salvianolic acid B and danshensu with mobile phase of acetonitrile(A)-1% formic acid solution(B) for gradient elution(0-2 min,18% A;2-3 min,18% -30% A;3-6 min,30% A) and detection wavelength at 280 nm.Equilibrium dialysis method was used to investigate binding rates of salvianolic acid B and danshensu with different plasma proteins. Result: Linear ranges of salvianolic acid B and danshensu in 3 kinds of plasma were 2-200 mg·L^-1,and in dialysis filtrates were 0.5-20 mg·L^-1.Mean protein binding rates of salvianolic acid B in rat,human plasma and BSA were 90.60%,92.25%,87.55%,respectively;these of danshensu were 35.44%,37.71%,33.49%,respectively. Conclusion: Binding rates of different concentrations of salvianolic acid B and danshensu in the same plasma have no significant difference,but binding rates of salvianolic acid B with 3 kinds of plasma protein are higher than danshensu.