| 紫金龙乙醇组分对脂多糖诱导的RAW264.7细胞分泌炎症因子的影响 |
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| 引用本文： | 张晓红,董莉,杨雅欣,刘星星,廖尚高,董永喜,李勇军,王爱民. 紫金龙乙醇组分对脂多糖诱导的RAW264.7细胞分泌炎症因子的影响[J]. 中国实验方剂学杂志, 2014, 20(21): 149-152 |
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| 作者姓名： | 张晓红 董莉 杨雅欣 刘星星 廖尚高 董永喜 李勇军 王爱民 |
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| 作者单位： | 贵阳医学院 药学院 贵州省药物制剂重点实验室, 贵阳 550004;贵阳医学院 药学院 贵州省药物制剂重点实验室, 贵阳 550004;贵阳医学院 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院 药学院 贵州省药物制剂重点实验室, 贵阳 550004;贵阳医学院 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院 民族药与中药开发应用教育部工程研究中心, 贵阳 550004;贵阳医学院 民族药与中药开发应用教育部工程研究中心, 贵阳 550004 |
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| 基金项目： | 国家科技支撑计划课题(2013BAI11B01);贵州省中药现代化项目(黔科合中药字[2011]5028号;黔科合中药字[2013]5062号);贵州省科技计划课题(黔科合LG字[2012]017号) |
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| 摘 要： | 目的:研究紫金龙乙醇组分(AVEC)对脂多糖(LPS)诱导小鼠单核巨噬细胞RAW 264.7分泌炎症因子的影响。方法:用脂多糖(10μg·L-1)刺激生长良好的RAW 264.7细胞24 h建立体外细胞炎症模型,以MTT法测定不同浓度AVEC对RAW 264.7细胞的毒性作用,Griess试剂法检测一氧化氮(NO)含量,ELISA法检测细胞上清液中肿瘤坏死因子α(TNF-α)和白介素6(IL-6)含量。结果:AVEC在低于400 mg·L-1时对RAW 264.7细胞无毒性作用。与空白对照组相比,LPS可以明显诱导RAW 264.7细胞分泌炎症因子TNF-α,IL-6和NO(P<0.01);与模型组相比,100~400 mg·L-1的AVEC可明显下调LPS诱导的RAW 264.7细胞释放炎症因子TNF-α,IL-6和NO(P<0.05,P<0.01),并呈现良好的剂量依赖关系。结论:AVEC可以抑制脂多糖诱导的RAW 264.7细胞炎症反应,其抗炎作用可能与减少炎症因子TNF-α,IL-6和NO有关。
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| 关 键 词： | 紫金龙 脂多糖 肿瘤坏死因子α 白介素6 |
| 收稿时间： | 2014-06-07 |
Effects of Ethanol Componentons from Aconitum vilmorinianum on Inflammatory Cytokines in LPS-stimulated RAW 264.7 Cell |
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| ZHANG Xiao-hong,DONG Li,YANG Ya-yin,LIU Xing-xing,LIAO Shang-gao,DONG Yong-xi,LI Yong-jun and WANG Ai-min. Effects of Ethanol Componentons from Aconitum vilmorinianum on Inflammatory Cytokines in LPS-stimulated RAW 264.7 Cell[J]. China Journal of Experimental Traditional Medical Formulae, 2014, 20(21): 149-152 |
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| Authors: | ZHANG Xiao-hong DONG Li YANG Ya-yin LIU Xing-xing LIAO Shang-gao DONG Yong-xi LI Yong-jun WANG Ai-min |
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| Affiliation: | Guizhou Provincial Key Laboratory of Pharmaceutics, School of Pharmacy, Guiyang Medical College, Guiyang 550004, China;Guizhou Provincial Key Laboratory of Pharmaceutics, School of Pharmacy, Guiyang Medical College, Guiyang 550004, China;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Guiyang Medical College, Guiyang 550004, China;Guizhou Provincial Key Laboratory of Pharmaceutics, School of Pharmacy, Guiyang Medical College, Guiyang 550004, China;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Guiyang Medical College, Guiyang 550004, China;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Guiyang Medical College, Guiyang 550004, China;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Guiyang Medical College, Guiyang 550004, China;Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, Guiyang Medical College, Guiyang 550004, China |
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