| 高效液相色谱法测定麻仁润肠丸中大黄素、大黄酚的含量 |
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| 引用本文： | 朱红,徐晓艳,庄妍. 高效液相色谱法测定麻仁润肠丸中大黄素、大黄酚的含量[J]. 环球中医药, 2009, 2(6): 439-441 |
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| 作者姓名： | 朱红 徐晓艳 庄妍 |
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| 作者单位： | 江西省药物研究所,南昌,330029 |
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| 摘 要： | 目的建立了麻仁润肠丸中大黄素、大黄酚含量测定方法。方法采用BDS C18柱,以甲醇-0.1%磷酸（85：15）,流速为1.0 ml/min,检测波长为254nm,外标法定量。结果发现大黄素在进样量0.03508～0.3508μg范围内,大黄酚在进样量0.09304～0.8304μg呈良好的线性关系;平均回收率大黄素为98.32%,RSD为1.05%（n=6）,大黄酚为97.98%,RSD为0.46%（n=6）。结论该方法快速简便,结果准确,可用于麻仁润肠丸的质量控制。
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| 关 键 词： | 麻仁润肠丸 高效液相色谱法 大黄素 大黄酚 含量测定 |
Determination of Emodin and Chrysophanot in Marenrunchang-pills by HPLC |
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| ZHU Hong,XU Xiao-yan,ZHUANG Yan. Determination of Emodin and Chrysophanot in Marenrunchang-pills by HPLC[J]. Global Chinese Medicine, 2009, 2(6): 439-441 |
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| Authors: | ZHU Hong XU Xiao-yan ZHUANG Yan |
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| Affiliation: | .( Jiangxi Institute of Materia Medica, Nanchang 330029, China ) |
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| Abstract: | Objective This paper reports a new method for the determination of emodin and ehrysophanol in Marenrunchang-pills. Method BDS C18 was used as the column, and the mobile phase was methanol -0.1% phosphorieacid（ 85：15 ）with a flow rate of 1 ml/min. The detection wavdlength was 254 nm. Results The calibration curve was in good linearity of emodin over the ranges of 0. 03508 - 0. 3508 μg and chrysophanol over the ranges of 0. 09304 - 0. 8304 ixg; the average recovery of emodin was 98. 32% with RSD 1.05% （ n = 6）, of chrysophanol was 97.98% with RSD 0.46% （ n = 6 ）. Conclusion This method is simple, rapid, accurate, and can be used for quality control of Marenrunchang-pills. |
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| Keywords: | Maren Runchang pill HPLC Emodin Chrysophanol Determination |
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