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| 聚合酶链反应方法检测急性白血病p15基因高度甲基化 | |
| 引用本文： | 陈飞,曾宪昌. 聚合酶链反应方法检测急性白血病p15基因高度甲基化[J]. 中华血液学杂志, 2000, 21(5): 250-252 |
| 作者姓名： | 陈飞 曾宪昌 |
| 作者单位： | 湖北医科大学附属第二医院血液科 |
| 摘 要： | 目的 探讨ｐ１５基因高度甲基化在急性白血病发病中的作用。方法 用甲基化敏感的限制性核酸内切酶消化结合聚合酶链反应技术。结果 在３６例急性白血病患中有２２例（６１．１％）存在ｐ１５基因高度甲基化，其中２６例急性非淋巴细胞白血病（ＡＮＬＬ）有１７（６５．４％），１０例急性淋巴细胞白血病有５例（５０．０％）存在ｐ１５基因高度甲基化。结合临床资料分析，有ｐ１５基因甲基化的ＡＮＬＬ患有较高的外周血白细胞 |
| 关 键 词： | 急性白血病 p15基因 高度甲基化 聚合酶链反应 |
| 修稿时间： | 1999-07-05 |
Detection of hypermethylation of p15 gene in patients with acute leukemia by polymerase chain reaction |
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| CHEN Fei,ZENG Xianchang. Detection of hypermethylation of p15 gene in patients with acute leukemia by polymerase chain reaction[J]. Chinese Journal of Hematology, 2000, 21(5): 250-252 | |
| Authors: | CHEN Fei ZENG Xianchang |
| Affiliation: | Department of Hematology, the Second Affiliated Hospital, Hubei Medical University, Wuhan 430071, China. |
| Abstract: | Objective To explore the role of hypermethylation of p15 gene in the pathogenesis of acute leukemias.Methods DNA from 36 cases of acute leukemia were investigated using restriction enzyme digestion combined with polymerase chain reaction(PCR) technique.Results Hypermethylations of p15 gene were found in 22 (61.1%) patients,including 17 cases of acute non lymphoblastic leukemia(ANLL) and 5 of acute lymphoblastic leukemia (ALL).ANLL patients exhibited p15 gene hypermethylations had higher WBC in peripheral blood (P<0 05) and higher percentages of leukemia cells in bone marrow(P<0.01).Conclusion Inactivation of p15 gene due to hypermethylation may be involved in the pathogenesis of some acute leukemias. |
| Keywords: | Gene p15 Hypermethylation Leukemia acute Polymerase chain reaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |