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| 脑红蛋白高表达对创伤性脑损伤的保护作用 | |
| 引用本文： | 冯兴军,尚爱加,周定标,张红红,韩志涛,胡亚卓. 脑红蛋白高表达对创伤性脑损伤的保护作用[J]. 临床神经外科杂志, 2013, 0(6): 337-340 |
| 作者姓名： | 冯兴军 尚爱加 周定标 张红红 韩志涛 胡亚卓 |
| 作者单位： | 武警总医院神经外科;中国人民解放军总医院神经外科;中国人民解放军老年医学研究所; |
| 基金项目： | 国家自然科学基金资助项目(30571903) |
| 摘 要： | 目的 观察脑红蛋白（Ngb）高表达对创伤性脑损伤有无保护作用.方法 将重组Ngb腺病毒载体立体定向注入大鼠脑内,使Ngb高表达,然后以Feeney＇s自由落体方法施以局灶性中度脑损伤,通过TTC染色和HE染色观察创伤性脑损伤程度是否减轻.结果 Ngb高表达组创伤性脑损伤灶体积较对照组明显减小,而且伤侧海马CA1区细胞丢失减少,凋亡细胞数明显减少.结论 脑组织内高表达Ngb可以减轻创伤性脑损伤的程度,即Ngb对创伤性脑损伤具有神经保护作用. |
| 关 键 词： | 脑红蛋白 创伤性脑损伤 腺病毒载体 神经保护剂 |
Effects of neuroglobin overexpression on traumatic brain injury |
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| Affiliation: | FENG Xing-jun, SHANG Ai-jia, ZHOU Ding-biao, et al. Department of Neurosurgery, General Hospital of Chinese People s Armed Police Forces, Beijing 100039, China |
| Abstract: | Objective To explore whether Neuroglobin （Ngb） protects brain from traumatic injury. Methods Rats were stereotaxically injected with recombinant adenovirus vector containing GFP-tagged Ngb （Ad-GFP-Ngb） into cortex and hippocampus, five days later, moderate traumatic brain injury was to be implemented by means of Feeney＇ s weight-dropping method, the changes of injured brain pathology were assessed by TYC and HE stain. Results The volume of injured brain of Ngb overexpressed group was decreased distinctly compared with that of control group, as well as cell loss and apoptosis in CA1 was improved significantly. Conclusions Over expression of Ngb may reduce the traumatic brain injury damage. |
| Keywords: | neuroglobin traumatic brain injury adenovirus vector neuroprotective agents |
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