A method for simultaneous recording of electrical activities and spectrophotometric signals from brain slice preparations in vitro |
| |
Authors: | Takanori Fujii Ken'ichi Toita |
| |
Affiliation: | (1) Kyoto Municipal Jr. College of Nursing, I-2 Higashitakada, Mibu, Nakagyo-ku, 604 Kyoto, Japan;(2) Kyoto City Institute of Public Health, I-2 Higashitakada, Mibu, Nakagyo-ku, 604 Kyoto, Japan |
| |
Abstract: | Organ spectrophotometry has been applied to analyze cytochrome redox changes in brain slice preparations. An interface-chamber method for maintaining metabolism of brain slice tissues was devised to reduce noise on recording traces of spectrophotometric signals, and then used for continuous monitoring and simultaneous recording of electrical and optical signals from brain slices. With this method, the noise level during the recording of redox states of cytochromes was decreased to 0.0004 A unit. |
| |
Keywords: | Interface chamber method Brain slice spectrophotometry Simultaneous recording of potential and redox state |
本文献已被 SpringerLink 等数据库收录! |
|