电针穴位对肠缺血-再灌注大鼠回肠内AChE活性的影响

目的 探讨电针穴位对肠缺血-再灌注大鼠回肠内AChE活性的影响。方法 采用夹闭大鼠肠系膜上动脉造成肠缺血-再灌注损伤模型,以改良Ellman法测定AChE,每日电针双侧“百会”、“风池”、“大钟”及“足三里穴”30min,持续7d和14d,波频率2—20Hz,强度2．0A。结果 实验后第7d,大鼠回肠内的AChE活性在缺血-再灌注组明显低干假手术组,电针组的活性则基本恢复;第14d,缺血-再灌注及电针组的AChE活性恢复。结论 肠缺血-再灌注所致的肠损伤可能与肠内AChE活性有关,电针能提高大鼠回肠内的AChE活性,从而可能对抗肠缺血-再灌注所致的肠损伤作用。

Influence of Electroacupuncture Points on AchE Activity of Ileum by Intestinal Ischemia-Reperfusion Rats

Objective To investigate the influence of electroacupuncture points on ileum acetylcholinesterase (AChE) activity of intestinal ischemia-reperfusion rats.Methods The model was established by occlusion of superior mesentery artery and the AChE activity of animals was detected by modifying Ellman method,and electroacupuncture (with frequency of 2-20Hz and intensity of 2.0A) on bilateral points of "Baihui", "Fengchi", "Dazhong" and "Zusanli" for 30 mim,every-day and last 7 and 14 days.Results At 7th day after experiment,in ileum,AChE activity of ischemia-reperfusion group was significantly decreased compared with sham-operetion group, but that of electroacupuncture group was recovered.At 14th day,AChE activity of ileum in ischemia-reperfusion group was lower than Sham-operation group, but in electroacupuncture group, it was recovered too.Conclusion Decreasing of AChE activity in ileum may relate to intestinal ischemia-reperfusion injury;Electroacupuncture can improve the AChE activity.