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The efficiency of interference of Potato virus X infection depends on the target gene |
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| Authors: | Takahashi Shuichiro Komatsu Ken Kagiwada Satoshi Ozeki Johji Mori Takuma Hirata Hisae Yamaji Yasuyuki Ugaki Masashi Namba Shigetou |
| Affiliation: | Laboratory of Plant Pathology, Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan. |
| Abstract: | RNA silencing is a natural defense response against viral infection. This phenomenon has been used to interfere with viral infections by exploiting fragments of viral genomes as sources of RNA silencing. Agrobacterium-mediated transient expression of a hairpin RNA derived from the TGBp1 gene of Potato virus X (PVX) induced RNA silencing of the TGBp1 gene and resulted in interference of PVX infection. The interference was induced in the infiltrated leaves but not in the upper non-infiltrated leaves. Transient expression of a CP hairpin RNA also induced interference of PVX. The TGBp1 hairpin RNA showed more efficient interference of PVX infection than the CP hairpin RNA. |
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