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| 人食管鳞癌EC9706细胞线粒体DNA中ND1基因突变的检测及意义 | |
| 引用本文： | 刘宗文,冯天平,赵景志,田薇薇,韩娜,孙淼淼,娄欣,胡爱侠. 人食管鳞癌EC9706细胞线粒体DNA中ND1基因突变的检测及意义[J]. 肿瘤基础与临床, 2012, 25(6): 461-463 |
| 作者姓名： | 刘宗文 冯天平 赵景志 田薇薇 韩娜 孙淼淼 娄欣 胡爱侠 |
| 作者单位： | 1. 郑州大学第二附属医院肿瘤科,河南,郑州,450014 2. 郑州大学公共卫生学院,河南,郑州,450001 3. 河南省肿瘤医院病理科,河南,郑州,450003 4. 郑州市中心医院,河南,郑州,450052 5. 商丘市中心医院,河南,商丘,476000 |
| 摘 要： | 目的通过对人食管鳞癌EC9706细胞线粒体DNA（mtDNA）中ND1基因进行检测，分析其基因突变的意义。方法培养人食管鳞癌EC9706细胞，提取其mtDNA中的ND1基因，并测序。结果测序发现，人食管鳞癌EC9706细胞mtDNA中ND1基因的3971处出现点突变，由C突变为T。结论人食管鳞癌EC9706细胞中mtDNA的ND1基因的突变与食管癌发生、发展关系密切。 |
| 关 键 词： | 人食管鳞癌 EC9706细胞 线粒体DNA ND1基因 |
Detection of Mutation in the Mitochondrial DNA ND1 Gene and Its Significance in the Human Esophageal Squamous Carcinoma EC9706 Cells |
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| Liu Zongwen , Feng Tianping , Zhao Jingzhi , Tian Weiwei , Han Na , Sun Miaomiao , Lou Xin , Hu Aixia. Detection of Mutation in the Mitochondrial DNA ND1 Gene and Its Significance in the Human Esophageal Squamous Carcinoma EC9706 Cells[J]. Journal of Basic and Clinical Oncology, 2012, 25(6): 461-463 | |
| Authors: | Liu Zongwen Feng Tianping Zhao Jingzhi Tian Weiwei Han Na Sun Miaomiao Lou Xin Hu Aixia |
| Affiliation: | 1.Department of Oncology,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China; 2.College of Public Health,Zhengzhou University,Zhengzhou 450001,China;3.Department of Pathology, Henan Tumor Hospital,Zhengzhou 450003,China;4.Zhengzhou Central Hospital,Zhengzhou 450052,China; 5.Shangqiu Central Hospital,Shangqiu 476000,China) |
| Abstract: | Objective To study the mutation of the mitochondrial DNA （mtDNA） ND1 gene of the human e- sophageal squamous carcinoma EC9706 cells. Methods The mtDNA was extracted in human esophageal squamous carcinoma EC9706 cells and ND1 gene was amplified and sequenced. Results The gene sequencing was success- fully detected. Point mutation （ C3 971T） in ND1 gene was found by sequencing in the human esophageal squamous carcinoma EC9706 cells. Conclusion The mutation of the mtDNA ND1 gene of the human esophageal squamous carcinoma EC9706 ceils is related to the occurrence and development of esophageal carcinoma. |
| Keywords: | human esophageal squamous carcinoma EC9706 cells mitochondrial DNA ND1 gene |
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