链脲佐菌素加膳食高脂高糖饲料诱导的2型糖尿病大鼠模型的验证

目的：验证链脲佐菌素（STZ）加高脂高糖饲料诱导的2型糖尿病大鼠模型的可靠性。方法：50只Wistar大鼠适应性饲养1w，随机分为两组，普通饲料组20只和高脂高糖组30只。普通饲料组以普通饲料喂养，高脂高糖组先喂以高脂高糖饲料6W，由尾静脉采血测空腹血糖（FBG）和胰岛素（FINS），并计算胰岛素敏感指数；然后按30mg／Kg腹腔注射1％的STZ溶液，1次/w，连续腹腔注射2次，1w后测血糖。以连续2次空腹血糖≥7.8mmol／L为造模成功，共25只。两组大鼠于高脂高糖组大鼠给予STZ后第2周、第4周测定空腹血糖和口服糖耐量。结果：与普通饲料组比较，饲养6W后高脂高糖组大鼠胰岛素水平升高（P〈0.05），高脂高糖组大鼠胰岛素敏感指数下降（P〈0.01）；给予STZ后2w高脂高糖组大鼠空腹血糖、2h血糖较普通饲料组升高（P〈0.01）：给予STZ 4W后空腹血糖、1h血糖、2h血糖较普通饲料组大鼠升高（P〈0.01）。结论：STZ加高脂高糖膳食诱导的2型糖尿病大鼠血糖升高，口服糖耐量异常，出现了明显的胰岛素抵抗，符合2型糖尿病的诊断标准。这种模型可靠，理想，简单，费用低，容易广泛推广。

Validation of Type 2 Diabetes Rat Model Induced by STZ Plus High Calorie and High Sugar Diet

Objective:To validate the reliability of type 2 diabetes rat model induced by streptozotocin (STZ)plus high calorie and high sugar diet. Methods:50 Wistar rats were divided randomly into two groups:high calorie and high sugar diet group(H group,n=30)and common diet group(C group,n=20). The levels of fasting blood glucose and insulin were detected and insulin sensitivity index was calculated on the sixth week after feed. At the same time,rats in H group were injected intraperitoneally 0.1% STZ at 30 mg/Kg on...