致病性钩端螺旋体微量DNA的聚合酶链反应(PCR)

本研究首次通过对黄疸出血群钩端螺旋体 DNA 的分子克隆的反复筛选,获得了两个重组DNA片段,其中一个具有广泛针对各群致病性钩体的特性,另一个仅对黄疸出血群钩体起杂交反应。经 DNA 序列分析,分别合成两对 PCR 寡核苷酸引物——Primer B1.2和 Primer B3.4。本研究证明 PCR 是一种灵敏度极高,针对性很强的 DNA 检测技术,可用于钩体病早期诊断和流行病学分类鉴定。

The Detection of DNA in Microquantity in Pathogenetic Leptospires by Polymerase Chain Reaction (PCR)

The genomic banks of icterohaemorrhagiae leptospira have been constructed firstly in this study and two recombinant DNA clones were selected by the means of molecular cloning, one of them can hybridized with most of pathogenetic leptospires, another can only hybridize with the serogroup icterohaemorrhagiae leptospira specially. Two pairs of polymerase chain reaction (PCR) oligonucleotied primers were synthesized by Applied Biosystems DNA Synthesier, LKB after the DNA sequence was analyzed, named Primer B 1. 2 and Primer B 3. 4. The study has demonstrated, therefore, that PCR is powerful detection techniques with highly sensitive and specific character, and could be developed into modern tool in early diagnosis and identification and analysis of epidemiology in Leptospirosis.