豚鼠胆汁中胃肠激素含量及其在成石过程中的变化

为探讨鼠胆汁中胃动素，血管活性肠肽和生长抑素的来源，释放调节及其在胆囊结石形成中的作用，采用放射免疫分析方法分别测定了正常对照组，致石组及恢复组豚鼠胆汁，胆囊壁组织及门静脉血中ＭＴＬ、ＶＩＰ和ＳＳ的含量以及胆汁理化性质变化。

GASTROINTESTINAL HORMONES IN GUINEA PIG BILE AND ITS CHANGES DURING GALLSTONE FORMATION

To investigate the origin and releasing relation of motilin (MTL), vasoactive intestinal peptide (VIP) and somatostatin (SS) in guinea pig bile as well as its effects during gallstone formation. Guinea pig were divided into three groups: control group (50 animals), on normal diet; lithogenic group (70 animals), fed with low protein low fat; and recovering group (50 animals), fed with low protein low fat and recovering normal food after the experiment of gallstone formation. MTL, VIP and SS in the bile gallbladder tissue and portal vein plasma of the normal control group were measured with radioimmunoassay. Meanwhile the changes of the gut peptides in the bile and the bile components from different groups were also compared. Results: In control group the levels of MTL, VIP and SS in the bile were higher than those in the plasma, but, obviously lower than those in the tissues, the concentration relationship between in the bile and in the tissue was a positive correlation. In contrast to the control group, MTL concentration decreased but VIP and SS increased in the bile of the lithogenic group, the physicochemical nature of the bile also became lithogenic. In the recovering group the bile also became lithogenic, but, the concentration of those peptides and the nature of the bile all got normal. Conclusion: MTL, VIP and SS in guinea pig bile originate mainly form the gallbladder wall tissues. Food components affect the levels of the gut peptides in bile, which promote the bile lithogenic changes and gallstone formation.