马鞭草总黄酮对HepG-2细胞增殖及侵袭力影响

目的 探讨马鞭草总黄酮（TFV）对肝癌HepG-2细胞增殖和侵袭力的影响。方法 实验设对照组、TFV 50、100、200 mg/L组，噻唑蓝法检测人肝癌HepG-2细胞增殖率，Hoechest 33342染色检测HepG-2细胞凋亡情况，Transwell法检测HepG-2细胞侵袭力变化，ELISA法测定HepG-2细胞中基质金属蛋白酶9（MMP-9）及血管内皮生长因子（VEGF）表达水平。结果 各剂量TFV均能明显抑制HepG-2 细胞生长，呈现剂量依赖性（P < 0.05）；与对照组比较，TFV 50、100、200 mg/L组HepG-2细胞凋亡率[分别为（10.31±0.51）%、（16.02±0.63）%、（35.05±1.12）%]明显增加，透膜细胞数[分别为（119.2±17.1）、（98.5±13.5）、（54.8±6.4）个]明显下降，差异均有统计学意义（P < 0.05）；与对照组比较，TFV 50、100、200 mg/L组HepG-2细胞MMP-9和VEGF表达水平[分别为（7.81±0.48）、（6.28±0.41）（3.08±0.35）和（0.87±0.07）、（0.63±0.04）、（0.45±0.06）]明显降低，差异有统计学意义（P < 0.05）。结论 马鞭草总黄酮可明显抑制人肝癌HepG-2细胞增殖、降低HepG-2细胞侵袭力，其机制可能与下调HepG-2细胞内MMP-9和VEGF表达水平有关。

Impact of total flavonoids of Verbena officinalis L on proliferation and invasiveness of human hepatocellular carcinoma HepG-2 cells

Objective To explore the effect of total flavonoids of Verbena officinalis L (TFV)on proliferation and invasiveness of human hepatocellular carcinoma (HepG-2) cells. Methods HepG-2 were treated with TFV at dosages of 50, 100, and 200 mg/L;the proliferation of HepG-2 cells was assayed by using 3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) method and the apoptosis of the cells was detected with Hoechest 33342;the invasiveness of the cells was detected with Transwell.The expressions of matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) were assessed with enzyme-linked immunosobent assay (ELISA). Results Compared with thecontrol group, different concentration of TFV could inhibit the growth of HepG-2 cells significantly (P < 0.05) and significantly promote apoptosis of the cells, with the apoptosis rates of 10.31±0.51%, 16.02±0.63%, and 35.05±1.12% and the invasiveness rates of 119.2±17.1, 98.5±13.5, and 54.8±6.4 for the HepG-2 cells with the TFV dosages of 50, 100, and 200 mg/L, respectively(all P < 0.05).The expression levels of MMP-9 (7.81±0.48, 6.28±0.41, and 3.08±0.35) and VEGF (0.87±0.07, 0.63±0.04, and 0.45±0.06) were down-rugulated for the HepG-2 cells with the TFV dosages of 50, 100, and 200 mg/L, respectively (all P < 0.05). Conclusion Total flavonoids of Verbena officinalis L can inhibit the proliferation of HepG-2 cells and reduce invasiveness of the cells, and the mechanism of the effect may be related to down-regulation MMP-9 and VEGF.