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| 氯化三丁基锡致巨噬细胞凋亡作用 | |
| 引用本文： | 曾怀才, 贺庆芝, 陈锋. 氯化三丁基锡致巨噬细胞凋亡作用[J]. 中国公共卫生, 2010, 26(1): 84-85. DOI: 10.11847/zgggws2010-26-01-45 |
| 作者姓名： | 曾怀才 贺庆芝 陈锋 |
| 作者单位： | 1.南华大学公共卫生学院劳动卫生与环境卫生学教研室, 湖南衡阳421001;;2.南华大学生命科学与技术学院生物学系;;3.南华大学公共卫生学院营养与毒理学教研室 |
| 基金项目： | 湖南省教育厅科研基金 |
| 摘 要： | 目的 探讨氯化三丁基锡(tributyltin chloride,TBTCl)致巨噬细胞株RAW264.7细胞的凋亡作用。方法 当RAW264.7细胞处于代数生长期时,分别加入不同浓度(0.0,0.025,0.05,0.10,0.20,0.40mg/L)的TBTCl处理48h,分别用四甲基偶氮噻唑蓝(MTT)法检测细胞活性,观察TBTCl对RAW264.7细胞增殖影响;吖啶橙/溴化乙锭(AO/EB)染色法观察凋亡细胞的形态,流式细胞仪检测TBTCl对RAW264.7细胞的凋亡率。结果 TBTCl能抑制RAW264.7细胞的增殖,其半数抑制浓度(IC50)=0.179mg/L;TBTCl也能诱导细胞的凋亡,当TBTC1达到0.40mg/L时,细胞凋亡率达到19.5%。结论 TBTC1可诱导细胞凋亡。 |
| 关 键 词： | 氯化三丁基锡 RAW264.7细胞 细胞凋亡 |
| 收稿时间： | 2009-05-21 |
Apoptosis of RAW 264.7 cells induced by tributyltin chloride |
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| ZENG Huai-cai, HE Qing-zhi, CHEN Feng. Apoptosis of RAW 264.7 cells induced by tributyltin chloride[J]. Chinese Journal of Public Health, 2010, 26(1): 84-85. DOI: 10.11847/zgggws2010-26-01-45 | |
| Authors: | ZENG Huai-cai HE Qing-zhi CHEN Feng |
| Affiliation: | 1.Department of Occupational and Environmental Health, Public Health School, South China University, Hengyang 421001, China |
| Abstract: | Objective To explore the apoptosis effect of tributyltin chloride (TBTC1)on RAW264.7 cells.Methods RAW264.7 cells were incubated with various concentrations of TBTCL for 24 hours.The cell growth inhibition was mea-sured by MTT,and the cell apoptosis was observed by flow cytometry analysis and fluorescence microscope.Results TBTCl could result in the descent of cell activity (IC50=0.179 mg/L) and could induce the apoptosis in RAW264.7 cells.The rate of apoptosis of RAW264.7 cells reached to 19.5% at TBTCl co... |
| Keywords: | tributyltin chloride RAW264.7 cell cell apoptosis |
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