豚鼠耳蜗损伤对耳蜗核神经元细胞面积的影响

为了解耳蜗损伤对中枢听觉系统形态和功能的改变及在改变过程中的意义，采用计算机图像处理技术观察了５只新生和１３只成年豚鼠损伤耳蜗后不同时间腹侧耳蜗核神经元细胞面积的改变。发现新生豚鼠耳蜗损伤后２４小时前腹侧耳蜗核和后腹侧耳蜗核神经元细胞面积无明显改变，成年豚鼠损伤耳蜗后４、７、６０天前腹侧耳蜗核神经元细胞面积较对侧分别缩小２０．９３％、２５．７０％、２８．７２％，６０天组较正常对照组减小５１％；后腹侧耳蜗核损伤侧较对照侧分别缩小１７．５８％、２０．３０％、３８．５５％，６０天组较正常对照组减小３２．７５％。证实耳蜗损伤可迅速引起腹侧耳蜗核神经元细胞面积的改变，提示临床听力损失早期开始听神经刺激相当重要。

Effect of monaural cochlear ablation on cell areas of ventral cochlear nucleus neurous in neonatal and adult guinea pigs

The changes of the cross sectional areas of anteroventral cochlear nucleus (AVCN) and posteroventral cochlear nucleus (PVCN) were studied in neonatal and adult guinea pigs after monaural cochlear ablation with computer imaging analysis system.The cross sectional areas of neurons in AVCN and PVCN were not significantly changed after 24h of monaural cochlear ablation in neonatal guinea pigs. But the cell areas of AVCN neurons of ablated side were respectively reduced by 20.93%,25.70% and 28.72 % compared to non ablated side in 4d,7d ,60d after cochlear ablation,the cell areas of PVCN neurons were reduced by 17.58%,20.30% and 38.55% respectively. The area reduction of AVCN and PVCN neurons of ablated side in 60d group with monaural cochlear ablation were 51.00% and 32.75% relative to normal control group.Our results showed that cochlear ablation could results in rapid cell area reduction of VCN neurons in guinea pigs.Our investigation implied that it is important to stimulate the auditory nerve early in the patients with hearing loss.