新型漏声表面波生物传感器液相检测条件的实验研究

目的探讨漏声表面波生物传感器液相检测系统的影响因素.方法构建检测和参比双路延迟线,并观察在不同影响因素如离子浓度、缓冲液pH值及不同粘度的甘油PBS溶液中两路延迟线的相位变化规律.结果检测延迟线声表面波信号的相位值随溶液粘度、离子浓度、pH值的增大而发生明显的变化,而参比通道的相位曲线变化不明显.＃结论成功构建了漏声表面波生物传感器液相检测检测技术平台,并探索了溶液的粘度、离子浓度、pH等因素对传感器的影响.

Test of a new style leaky surface acoustic wave biosenser in liquid phase

Objective To investigate the influential factors in the detection system of the leaky surface acoustic wave biosensor in liquid phase.Methods A detection delay line and a reference delay line were constructed and the phase of the two delay line changes was evaluated in different ion concentration,pH value,and glycerol PBS at different viscosities. Results The phase of the detection delay line had marked changes in accordance with the increase of viscosity,ion concentration and pH value, and the reference delay line had no marked change.Conclusion The detection system of the leaky surface acoustic wave biosensor in liquid phase was designed and the influence of the viscosity,ion concentration and pH value to the leaky surface acoustic wave biosensor was investigated.