| TaqMan荧光定量逆转录-聚合酶链反应快速检测麻疹病毒核酸 |
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| 引用本文： | 卢亦愚,严菊英冯燕史雯,茅海燕. TaqMan荧光定量逆转录-聚合酶链反应快速检测麻疹病毒核酸[J]. 中国疫苗和免疫, 2005, 11(1): 1-4 |
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| 作者姓名： | 卢亦愚 严菊英冯燕史雯 茅海燕 |
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| 作者单位： | 卢亦愚（浙江省疾病预防控制中心,杭州,310009）;严菊英（浙江省疾病预防控制中心,杭州,310009）;冯燕（浙江省疾病预防控制中心,杭州,310009）;史雯（浙江省疾病预防控制中心,杭州,310009）;茅海燕（浙江省疾病预防控制中心,杭州,310009） |
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| 摘 要： | 目的建立以特异性荧光探针为特点的TaqMan荧光定量逆转录-聚合酶链反应(RT-PCR)方法.方法检测麻疹病毒的核酸,对设计的引物与探针进行筛选与条件优化.结果 Tag Man荧光定量RT-PCR方法具有对麻疹病毒核酸检测的高度特异性与准确性,检测的敏感度可达0.1TCID50,从病毒核酸提取至检测完成仅需3个多小时,操作简便,而且大大减少了常规PCR扩增产物的污染机会.结论采用Tag Man荧光定量RT-PCR方法对麻疹病毒与相关的临床样本进行了检测,均获得了满意的结果,为麻疹病毒的分子生物学检测,提供了一种新方法.
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| 关 键 词： | 荧光定量聚合酶链反应 TaqMan探针 麻疹病毒 检测 |
| 文章编号： | 1006-916X(2005)01-0001-04 |
| 修稿时间： | 2004-07-07 |
TaqMan-based Real-time PCR Assay for Quick Detection of Measles Virus RNA |
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| LU Yi-yu,YAN Ju-ying,FENG Yan,et al. TaqMan-based Real-time PCR Assay for Quick Detection of Measles Virus RNA[J]. Chinese Journal of Vaccines and Immunization, 2005, 11(1): 1-4 |
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| Authors: | LU Yi-yu YAN Ju-ying FENG Yan et al |
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| Abstract: | Objective To establish a TaqMan-based real-time PCR assay for detection of measles virus RNA,the specific primers and probes were selected and the reactive condition was optimized. Methods The specificity of the assay was high and the sensitivity of the assay was 0.1TCID_(50).The course of extraction of the measles virus RNA and the real-time PCR only took three hours. Results It had the advantages of contamination control,automation,and direct virus quantification.The applicability of this method was evaluated with measles virus and clinical samples and the results was satisfied. (Conclusion )This TaqMan-based real-time PCR was a reliable,sensitive,specific tool for molecular diagnosis of measles virus. |
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| Keywords: | Real-time PCR TaqMan-based probe Mealsles virus Detection |
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