| 青藤碱拮抗体外培养的乳鼠心肌细胞自由基损伤 |
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| 引用本文： | 李乐,高晓利,袁秉祥. 青藤碱拮抗体外培养的乳鼠心肌细胞自由基损伤[J]. 中国医疗前沿, 2007, 0(8) |
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| 作者姓名： | 李乐 高晓利 袁秉祥 |
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| 作者单位： | 浙江工业大学药学院，西安交通大学医学院药理教研室，西安交通大学医学院药理教研室 浙江杭州 310014，陕西西安 710061，陕西西安 710061 |
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| 摘 要： | 目的研究青藤碱(Sin)对体外培养乳鼠心肌细胞自由基损伤的保护作用。方法应用O_2~-诱导培养乳鼠心肌细胞损伤模型,通过测定乳酸脱氢酶(LDH)活性、MTT代谢率、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量等指标,观察Sin对心肌细胞自由基损伤的保护作用。结果与损伤组比较,Sin保护组的LDH释放量显著减少(P＜0．01);MTT代谢率显著增高(P＜0．01);Sin明显抑制损伤率;SOD活性明显增高;MDA含量明显降低(P＜0．01)。结论Sin通过清除氧自由基保护O_2~-损伤的心肌细胞。
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| 关 键 词： | Sin 心肌细胞培养 自由基 乳酸脱氢酶 丙二醛 |
Effect of Sinomenine Against Cultured Neonatal Rat Cardiomyocytes Injured by Free Radicals |
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| LI Le GAO Xiao-li YUAN Bing-xiang .School of Pharmacy,Zhejiang University of technology Hangzhou China. Effect of Sinomenine Against Cultured Neonatal Rat Cardiomyocytes Injured by Free Radicals[J]. China Healthcare Innovation, 2007, 0(8) |
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| Authors: | LI Le GAO Xiao-li YUAN Bing-xiang .School of Pharmacy Zhejiang University of technology Hangzhou China |
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| Affiliation: | LI Le~1 GAO Xiao-li~2 YUAN Bing-xiang~2 1.School of Pharmacy,Zhejiang University of technology Hangzhou 310014 China, 2.Department of Pharmacology in Xi,an medical University Xi,an 710061 China |
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| Abstract: | Objective To study the protective effect of sinomenine on the cultured neonatal rat cardiomyocytes injured by free radicals.Methods Injury model of the cultured neonatal rat cardiomyocytes was erected by super oxygen anion(O_2~-).The activity of lactic dehydrogenase(LDH),MTT metabolic rate,Superoxide Dismutases(SOD),cellular melondadehyde(MDA)were determined to observe sinomenine effect on cultured neonatal rat cardiomyocytes injured by free radicals.Results Compared with the model group,sinomenine significantly decreased the release of LDH,lowered the content of MDA and markedly increased the activity of SOD,increased MTT metabolic rate.Conclusion sinomenine has the protective effect by eliminating free radicals on cuhured neonatal rat cardiomyocytes O_2~--induced injury. |
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| Keywords: | sinomenine free radicals cultured cardiac myocyte LDH MDA |
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