人免疫缺陷病毒HB-J株的分离和生物学特性研究

目的：分离和鉴定我国流行的人免疫缺陷病毒（ｈｕｍａｎ ｉｍｍｕｎｏｄｅｆｉｃｉｅｎｃｙ ｖｉｒｕｓ，ＨＩＶ）毒株，研究我国ＨＩＶ毒株的生物学特性。方法：周外用血单个核细胞（ｐｅｒｉｐｈｅｒａｌ ｂｌｏｏｄ ｍｏｎｏｎｕｃｌｅａｒ ｃｅｌｌｓ，ＰＢＭＣ）共培养的方法分离ＨＩＶ，用观察细胞病变、检测Ｐ２４抗原和前病毒ＤＮＡ的方法监测病毒生长的情况。结果：在建立共培养的第１２天观察到明显的细胞病变，主要表面为大泡样细胞和多核巨细胞。接种第９天细胞培养上清液的Ｐ２４抗原由阴性转为阳性，并且浓度迅速增加，接种第１２天浓度即达到１７８０ｐｇ／ｍｌ，第１４天达到峰值，为２３２０ｐｇ／ｍｌ。但该株病毒的ＰＢＭＣ培养上清液转种于ＭＴ４细胞后，培养上清液Ｐ２４抗原和前病毒ＤＮＡ始终为阴性且不出现细胞病变。结论：用ＰＢＭＣ共培养的方

Isolation of HIV HB-J strain and the observation of its biological characteristics

Objective:To isolate and identify the prevalent HIV strain in China.Methods:The HIV was isolated by co culture with PBMC taken from healthy donor and the virus was monitored by observation on cytopathic effect,P24 antigen and viral DNA. Results:The giant syncytia were observed 12 days after the inoculation. The P24 antigen turned to be detected on day 9 and its concentration in the supernatant of cell culture increased abruptly to 2 320 pg/ml 5 days later. The HB J strain could not infect the MT4 cell as indicated by the negative result of P24 antigen examination of the supernatant and the absence of cytopathological change. Conclusions: A human immunodeficiency virus strain named HB J was isolated by the PBMC co culture method.The difference of co receptor expressed on the surface of PBMC and MT4 may be the reason of the different susceptibility to infection by this HIV strain. [