| 脊髓小脑共济性失调2型家系永生细胞株的建立及遗传稳定性检测 |
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| 引用本文： | 林克勤,孙浩,张昌军,陶玉芬,易文,黄小琴,史荔,褚嘉祜. 脊髓小脑共济性失调2型家系永生细胞株的建立及遗传稳定性检测[J]. 中华医学遗传学杂志, 2009, 26(4). DOI: 10.3760/cma.j.issn.1003-9406.2009.04.003 |
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| 作者姓名： | 林克勤 孙浩 张昌军 陶玉芬 易文 黄小琴 史荔 褚嘉祜 |
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| 作者单位： | 1. 中国医学科学院北京协和医学院医学生物学研究所,昆明,650118
2. 湖北省十堰市人民医院生殖医学中心 |
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| 摘 要： | 目的 建立伴帕金森氏病症状的脊髓小脑共济性失调2型家系永生细胞株,提供永久性的实验研究材料.方法 采用EB病毒加入环胞霉素A的转化细胞技术,建立了该家系的永生细胞株.并检测传代细胞与家系血液中的微卫星标记有无改变,确定细胞株的遗传稳定性.结果 成功建立此家系25株永生细胞株,所检测的微卫星位点未发生变化.结论 所建永生细胞株染色体核型检测无异常,转化细胞株与血液中的微卫星的遗传稳定性无差异.
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| 关 键 词： | EB病毒 环胞霉素A 永生细胞株 伴帕金森氏病症状的脊髓小脑共济性失调2型 微卫星 遗传稳定性 |
Establishment of immortalized cell lines and genetic stability evaluation for a family with spinocerebellar ataxia type 2 |
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| LIN Ke-qin,SUN Hao,ZHANG Chang-jun,TAO Yu-fen,YI Wen,HUANG Xiao-qin,SHI Li,CHU Jia-you. Establishment of immortalized cell lines and genetic stability evaluation for a family with spinocerebellar ataxia type 2[J]. Chinese journal of medical genetics, 2009, 26(4). DOI: 10.3760/cma.j.issn.1003-9406.2009.04.003 |
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| Authors: | LIN Ke-qin SUN Hao ZHANG Chang-jun TAO Yu-fen YI Wen HUANG Xiao-qin SHI Li CHU Jia-you |
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| Abstract: | nes were established successfully from the family and the microsatellite markers were unchanged.Conclusion The karyotypes of the immortal cell lines were normal and the cell lines were genetically stable. |
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| Keywords: | Epstain Barr virus cyclosporine A immortal cell lines spinocerebellar ataxia type 2 with Parkinson disease symptoms microsatellites genetic stability |
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