肿瘤坏死因子单克隆抗体对小鼠肺炎链球菌感染的影响

给小鼠吸入肺炎链球菌同时在尾静脉注射肿瘤坏死因子单克隆抗体，动态检测感染后２４小时内血浆，气管肺泡灌洗液肿瘤坏死因子含量和肺病理的变化，发现肿瘤坏死因子单克隆抗体显著抑制血浆，气管肺泡灌洗液肿瘤坏死因子的升高，抑制白细胞降集，附壁，减轻白细胞对肺细胞的浸润，阻止肺实变的发生。肺炎链球菌及其产物可诱发肿瘤坏死因子释放，肿瘤坏死因子可能是肺炎链球菌感染组织损伤的主要介质。

Streptococcas pneumoniae-induced pulmonary consoli-dation prevenled with tumor necrosis factor alphamonoclonal anubody in mouse

treptococcus pnumoniae stimulate mouse peri-toneal macrophages to release tumor necrosis factor al-pha (TNFa) in vitro. When penicillin was added tolysate bacterium, TNFa release was accelarated. 150Kunming mice were infected with streptococcus pnumo-niae through inspiration. Dynamic changes of TNFaconcentration in serum and bronchoalveolar lavage fluidwere detected; pulmonary pathological changes were al-so observed. It was found that TNFa monoclonal anti-body significantly attennuates TNFa activity in serumand bronchoalveolar lavage fluid, white blood cellchemotaxis, emigation, and infiltration were inhibrted.We conclude that streptococcus pnumoniae infectionstimulates TNFa release, and TNFa is probabaly themajor mediator that causes tissue damage during strep-tococcus pnumoniae infection. As penicillin accelaratesTNFa release, singal therapy of penicillin may worsetissue damage.