| 改进ELISA法快速定量测定心肌肌钙蛋白Ⅰ方法学评价 |
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| 引用本文： | 刘兆军,刘海燕,秦卫胜. 改进ELISA法快速定量测定心肌肌钙蛋白Ⅰ方法学评价[J]. 医学检验与临床, 2003, 0(1) |
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| 作者姓名： | 刘兆军 刘海燕 秦卫胜 |
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| 作者单位： | 山东省东营市人民医院，山东省东营市人民医院，山东省东营市人民医院 东营 257091，东营 257091，东营 257091 |
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| 摘 要： | 目的 用改进ELISA法快速定量测定血中心肌肌钙蛋白I,以对该方法的方法学性能作一评价。方法 以临床血清标本作为检测标本,用改进的酶联免疫法测定血中的肌钙蛋白I,观察其灵敏度、线性试验为线性范围、精密度和回收率,并与进口产品比较。结果 测得灵敏度及0.05ng/ml。线性范围0.05-4ng/ml。低、高两种浓度的肌钙蛋白I批内变异分别为7.6％和6.8％;批间变异分别为9.0％和8.1％。与进口产品比较其相关系数为0.9754,P>0.05,结果无显著性差异。结论该方法有较高的灵敏度,重复性好,结果准确可靠,测量时间短等优点,能满足临床需要。
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| 关 键 词： | 酶联免疫法 心肌肌钙蛋白Ⅰ |
Quick detection of Serum CTnI with improved Enzyme - linking Immunoassay |
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| Liu Zhaojun,Liu Haiyan,Qin Weisheng People's Hospital of Dongying,Shandong Dongying China. Quick detection of Serum CTnI with improved Enzyme - linking Immunoassay[J]. Medical Laboratory Science and, 2003, 0(1) |
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| Authors: | Liu Zhaojun Liu Haiyan Qin Weisheng People's Hospital of Dongying Shandong Dongying China |
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| Affiliation: | Liu Zhaojun,Liu Haiyan,Qin Weisheng People's Hospital of Dongying,Shandong Dongying 257091 China |
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| Abstract: | ObjectiveDetection of serum CTnI With ELISA and to evaluate this methods. Methods Serum CTnI were detected with ELISA. Sensitivity,Linearity and Precision were evaluated and compared with the import, agent Results The sensitivity of CTnI wen; 0. 05ng; ml. linear range of CTnI were 0. 05 -4ng/ml. The within - run CV for CTnI were 7.6% and 6.8%,between- run CV were 9.0% and 8.1%. Compared 20 samples with import,CTnI of the relative coefficient r: 0. 9754 respectively, (P>0. 05) The results have no significant difference. Conclusion The assay has better sensitivity, reproducibility and accuracy and less measuring time. ELISA had a good relativity as import. It can act as methods of measuring CTnI. |
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| Keywords: | Enzyme- linkingimmunoassay CTnI |
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