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| 34例乳腺癌HER-2基因扩增与蛋白表达相关性研究 | |
| 引用本文： | 张元,吴宁,何小燕,阮思蓓,罗霞,唐明希. 34例乳腺癌HER-2基因扩增与蛋白表达相关性研究[J]. 中国医药科学, 2013, 0(20): 16-18 |
| 作者姓名： | 张元 吴宁 何小燕 阮思蓓 罗霞 唐明希 |
| 作者单位： | [1]泸州医学院病理教研室,四川泸州646000 [2]四川省德阳市人民医院病理科,四川德阳618000 |
| 基金项目： | 泸州医学院博士启动基金（2011204）. |
| 摘 要： | 目的了解乳腺癌组织中人表皮生长因子受体2（HER-2）基因扩增与Her-2／neu蛋白表达的一致性与相关性。方法采用荧光原位杂交（FISH）法及免疫组化（IHC）法分别检测349例浸润性乳腺癌患者的HER-2基因扩增与蛋白表达情况，分析二者检测结果的一致性与相关性。结果FISH与IHC符合率为55．3％，结果存在一致性（Kappa=0．189，P=0．000），呈正相关（r=0．434，P=0．000）。结论FISH与IHC两种检测结果存在一致性，但IHC（＋～＋＋＋）患者均应以FISH检测作为评价HER-2基因是否扩增的标准方法。 |
| 关 键 词： | 乳腺癌 人表皮生长因子受体-2 荧光原位杂交 免疫组织化学 |
Correlation between HER-2 gene amplification and Her-2/neu protein expression in 349 patients with invasive breast cancer |
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| Affiliation: | ZHANG Yuan WU Ning HE Xiaoyan RUAN Sibei LUO Xi TANG Ming( 1.Department of Pathology, Luzhou Medical College, Luzhou 646000, China; 2.Department of Pathology, People＇s Hospital of Deyang, Deyang 618000, China) |
| Abstract: | Objective To investigate the correlation between human epidermal growth factor receptor 2（HER-2） gene amplification and Her-2/neu protein expression in invasive breast cancer patients. Methods Using fluorescence in situ hybridization （FISH） and immunohistochemistry（IHC） method, the HER-2 gene amplification and Her-2/neu protein expression status were detected, respectively, and its concordance and correlation in a total of 164 samples of invasive breast cancer tissues. Results The overall coincidence rate of FISH and IHC was 55.3% （Kappa=0.189, P-0.000）, with positive correlation （r=0.434, P=0.000） between them. Conclusion There is a concordance between the Her-2/neu protein expression and its gene amplification. HER-2 gene amplification status should be further detected by FISH in patients with HER-2 protein expression （＋ ~ ＋＋＋）. |
| Keywords: | Breast cancer Human epidermal growth factor receptor 2 （HER-2） Fluorescence in situ hybridization（FISH） Immunohistochemistry （IHC） |
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