多(二磷酸腺苷-核糖)水解酶抑制剂单宁酸对糖尿病大鼠肾脏病变的影响及其机制

目的：研究多（二磷酸腺苷-核糖）水解酶（PARG）抑制剂单宁酸（GLTN）对糖尿病
大鼠肾脏病变的影响并探讨其可能的机制。方法：将实验动物随机分为正常对照组（NC组）、糖尿病组（DM组）、糖尿病3-氨基苯甲酰胺（3-AB）处理组（CDT组）、糖尿病GLTN低剂量（20 mg·kg-1·d^-1）处理组（LDT组）和糖尿病GLTN高剂量（30 mg·kg-1·d^-1）处理组（HDT组），12周后检查各组动物血糖、血肌酐、尿素氮、尿蛋白排泄率水平及肾脏病变情况，免疫组织化学法检测各组大鼠肾组织PARP阳性信号表达。结果：与DM组比较，LDT和HDT组血糖水平明显降低（P<0.01），血肌酐、尿素氮水平和尿蛋白排泄率也明显降低(P<0.01,P<0.05,P<0.01），LDT和HDT组糖尿病大鼠的肾脏病变程度减轻，肾组织PARP蛋白表达降低(P<0.05)。但LDT和HDT组之间上述肾功能参数比较差异无显著性(P>0.05)。结论：GLTN可降低糖尿病大鼠的血糖水平，抑制肾组织PARP表达，改善肾脏功能和形态异常。

Effect of poly(ADP-ribose)glycohydrolase inhibitor gallotannin on renal lesion in experimental diabetic rats and its mechanism

Objective To investigate the effect of poly(ADP-ribose)glycohydrolase(PARG)inhibitor gallotannin on renal lesion in STZ-induced diabetic rats and its mechanism.Methods Wistar rats were randomly divided into five groups:normal control (NC)group,STZ-induced diabetic (DM)group,diabetic rats treated with 3-aminobenzamide(CDT)group,diabetic rats treated with gallotannin 20and 30mg·kg-1·d-1 (LDT and HDT)groups.The levels of blood glucose,serum creatinine,blood urea nitrogen,urinary protein excretion were detected after treatment for 12weeks.The renal morphology was observed and the expression of poly(ADP-ribose) polymerase(PARP)was tested by immunohistochemical method.Results Compared with DM group,the levels of blood glucose,serum creatinine,blood urea nitrogen and urinary protein excretion were significantly decreased in both LDT and HDT groups(P<0.05or P<0.01).The renal lesion and the signal of positive expressing PARP in renal tissues were markedly reduced in diabetic rats treated with GLTN.But there were no significantdifferencesofthe indexes mentioned above between LDT and HDT groups(P>0.05).Conclusion Gallotannin can lower the level of blood glucose,inhibit the renal PARP expression,and ameliorate the renal functional and morphological abnormalities in diabetic rats.